Technical recommendations for analyzing oxylipins by liquid chromatography-mass spectrometry

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Schebb, Nils Helge ; Kampschulte, Nadja ; Hagn, Gerhard ; Plitzko, Kathrin ; Meckelmann, Sven W. ; Ghosh, Soumita ; Joshi, Robin ; Kuligowski, Julia ; Vuckovic, Dajana ; Botana, Marina T. ; Sanchez-Illana, Angel ; Zandkarimi, Fereshteh ; Das, Aditi ; Yang, Jun ; Schmidt, Louis ; Checa, Antonio ; Roche, Helen M. ; Armando, Aaron M. ; Edin, Matthew L. ; Lih, Fred B. ; Aristizabal-Henao, Juan J. ; Miyamoto, Sayuri ; Giuffrida, Francesca ; Moussaieff, Arieh ; Domingues, Rosario ; Rothe, Michael ; Hinz, Christine ; Das, Ujjalkumar Subhash ; Rund, Katharina M. ; Taha, Ameer Y. ; Hofstetter, Robert K. ; Werner, Markus ; Werz, Oliver ; Kahnt, Astrid S. ; Bertrand-Michel, Justine ; Le Faouder, Pauline ; Gurke, Robert ; Thomas, Dominique ; Torta, Federico ; Milic, Ivana ; Dias, Irundika H. K. ; Spickett, Corinne M. ; Biagini, Denise ; Lomonaco, Tommaso ; Idborg, Helena ; Liu, Jun-Yan ; Fedorova, Maria ; Ford, David A. ; Barden, Anne ; Mori, Trevor A. ; Kennedy, Paul D. ; Maxey, Kirk ; Ivanisevic, Julijana ; Gallart-Ayala, Hector ; Gladine, Cecile ; Wenk, Markus ; Galano, Jean-Marie ; Durand, Thierry ; Stark, Ken D. ; Barbas, Coral ; Garscha, Ulrike ; Gelhaus, Stacy L. ; Ceglarek, Uta ; Flamand, Nicolas ; Griffin, Julian L. ; Ahrends, Robert ; Arita, Makoto ; Zeldin, Darryl C. ; Schopfer, Francisco J. ; Quehenberger, Oswald ; Julian, Randall ; Nicolaou, Anna ; Blair, Ian A. ; Murphy, Michael P. ; Hammock, Bruce D. ; Freeman, Bruce ; Liebisch, Gerhard ; Serhan, Charles N. ; Koefeler, Harald C. ; Jakobsson, Per-Johan ; Steinhilber, Dieter ; Gelb, Michael H. ; Holcapek, Michal ; Andrew, Ruth ; Giera, Martin ; Fitzgerald, Garret A. ; Murphy, Robert C. ; Newman, John W. ; Dennis, Edward A. ; Ekroos, Kim ; Milne, Ginger L. ; Gijon, Miguel A. ; Vesper, Hubert W. ; Wheelock, Craig E. ; O'Donnell, Valerie B.

• Tipo de documento: Artigo Científico
• Fonte: SCIENCE SIGNALING; v. 18, n. 887, p. 3-pg., 2025-05-20.

Resumo

Several oxylipins are potent lipid mediators that regulate diverse aspects of health and disease and whose quantitative analysis by liquid chromatography-mass spectrometry (LC-MS) presents substantial technical challenges. As members of the lipidomics community, we developed technical recommendations to ensure best practices when quantifying oxylipins by LC-MS. (AU)

• Processo FAPESP: 13/07937-8 - Redoxoma
• Beneficiário: Ohara Augusto
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs