| Texto completo | |
| Autor(es): |
Zanoni, Jessica de Araujo
;
Zilli, Izabela Karolina Costa
;
Pretto, Guilherme de Paula
;
Seixas, Flavio Augusto Vicente
;
Lima, Marcela Marques de Freitas
;
Lemos, Eliana Gertrudes de Macedo
;
Gomes, Eleni
;
Zazeri, Gabriel
;
Bonilla-Rodriguez, Gustavo Orlando
Número total de Autores: 9
|
| Tipo de documento: | Artigo Científico |
| Fonte: | BIOTECH; v. 14, n. 3, p. 16-pg., 2025-08-18. |
| Resumo | |
Industrial applications of xylanases in high-temperature settings are limited by enzyme instability. This study evaluated glycerol and phenolic compounds as modulators of the catalytic and structural properties of a recombinant Myceliophthora heterothallica endoxylanase (rMhXyn) expressed in Komagataella phaffii. Glycerol (20% v/v) significantly improved thermostability (5-fold increase in half-life at 55 degrees C), decreased the activation energy for catalysis, and enhanced structural rigidity as evidenced by molecular dynamics simulations (reduced RMSD and Rg). In contrast, phenolic acids provided only short-term stabilization at moderate temperatures and did not confer structural benefits. Enzyme kinetics revealed that glycerol enhanced catalytic turnover (up arrow Vmax), while phenolic compounds modified both K ' and cooperativity (Hill coefficient). Thermodynamic analysis supported glycerol's stabilizing effect, with increased triangle H(D) and a positive shift in triangle S(D). These results suggest glycerol as a superior stabilizer for rMhXyn in high-temperature bioprocesses such as lignocellulosic biomass hydrolysis. These findings highlight the potential of targeted additives to improve enzyme performance for biotechnological applications. (AU) | |
| Processo FAPESP: | 19/20825-0 - Utilização de dados (meta)genômicos e consórcios microbianos para criação de coquetel enzimático para produção de metabólitos de interesse industrial através de biorrefinaria da biomassa lignocelulósica |
| Beneficiário: | Eliana Gertrudes de Macedo Lemos |
| Modalidade de apoio: | Auxílio à Pesquisa - Programa BIOEN - Temático |