| Texto completo | |
| Autor(es): |
Ribeiro Dos Santos Borges, Veronica
;
Caroline Ribeiro Araujo, Juliani
;
Nanni Vieira, Marilia
;
Adrielly Dias Da Cruz, Leticia
;
Falchete Do Prado, Renata
;
Barbosa, Lucas
;
Triches, Eliandra De Sousa
;
Campos, Tiago Moreira Bastos
;
Marotta Reis De Vasconcellos, Luana
Número total de Autores: 9
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Biomedical Materials; v. 21, n. 1, p. 18-pg., 2026-02-01. |
| Resumo | |
Osteoporotic bone presents a compromised regenerative niche, with reduced osteoblast function and an imbalance between bone formation and resorption, limiting the success of conventional defect filling strategies. Injectable biomaterials that conform to irregular defects and provide localized osteogenic cues are particularly relevant. Here, we developed injectable sodium alginate hydrogels (HPs) incorporating 45S5 bioactive glass (HB) or the same glass functionalized with two osteoporosis-relevant osteotropic drugs deliberately selected for distinct mechanisms of action: raloxifene hydrochloride (HBRx; selective estrogen receptor modulation) and strontium ranelate (HBSr; dual action on bone remodeling). Within the scope of this work, we established material feasibility and comparative in vitro performance through quantitative assessment of microstructure and surface behavior, together with cytocompatibility and osteogenic readouts. Glass incorporation remodeled the scaffold microstructure, increasing mean pore size from 54.0 +/- 17.9 mu m (HP) to 139.9 +/- 51.5 mu m (HB), while drug functionalization produced intermediate pores (84.8 +/- 26.3 mu m for HBRx; 85.5 +/- 37.4 mu m for HBSr). The increased inorganic contribution in the composites was reflected by higher residual mass at 800 degrees C (from 33.5% in HP to 45.0%-48.3% in glass-containing groups) and by shifts in wettability, with all formulations remaining hydrophilic (theta < 90 degrees) but differing between functionalizations (44.2 +/- 9.1 degrees for HBRx vs 62.4 +/- 11.8 degrees for HBSr). All HPs were cytocompatible (day 7 relative viability >= 70%) and supported osteogenic readouts (protein production, alkaline phosphatase activity, calcium deposition, and mineralized nodules), with HBSr showing the most favorable overall cellular response among the composites. Collectively, these findings indicate that osteoporosis-relevant drug-functionalized 45S5 within injectable alginate HPs provides a quantitative route to tune microstructure and interfacial behavior while preserving cytocompatibility and osteogenic potential. Future work will prioritize in vivo validation in osteoporotic models to assess bone repair efficacy and determine whether localized delivery mitigates drug-specific drawbacks associated with systemic therapies. (AU) | |
| Processo FAPESP: | 21/05274-8 - Sistema drug delivery em hidrogel, injetável e impressão 3D, para reparo ósseo em ratas ovariectomizadas |
| Beneficiário: | Luana Marotta Reis de Vasconcellos |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 23/16554-7 - Desenvolvimento de arcabouços celulares multicamadas contendo vidro bioativo com estrutura hierárquica de poros pela combinação das técnicas de eletrofiação e de manufatura aditiva para aplicações na medicina regenerativa |
| Beneficiário: | Veronica Ribeiro dos Santos Borges |
| Modalidade de apoio: | Bolsas no Brasil - Programa Fixação de Jovens Doutores |
| Processo FAPESP: | 22/03799-9 - Uma abordagem inovadora para o reparo ósseo associado à osteopenia com o uso de sistema drug delivery de hidrogéis injetáveis incorporados com biovidro funcionalizado com raloxifeno e ranelato de estrôncio. |
| Beneficiário: | Juliani Caroline Ribeiro de Araújo |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |
| Processo FAPESP: | 20/12874-9 - Infiltração gradada por vidro em um compósito experimental à base de whiskers de alumina e zircônia: desenvolvimento, caracterização microestrutural e das propriedades ópticas e mecânicas |
| Beneficiário: | Tiago Moreira Bastos Campos |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |
| Processo FAPESP: | 23/08439-3 - HIDROGEL COM SISTEMA DRUG DELIVERY DE RANELATO DE ESTRÔNCIO E RALOXIFENO, IMPRESSO EM 3D, PARA REPARO ÓSSEO EM RATAS OVARIECTOMIZADAS: estudo in vitro e in vivo. |
| Beneficiário: | Marília Nanni Vieira |
| Modalidade de apoio: | Bolsas no Brasil - Mestrado |