MICROSATELLITE LOCI FOR PASPALUM ATRATUM (POACEAE) AND CROSS-AMPLIFICATION IN OTHER SPECIES

Autor(es)

Cidade, F. W. ^[1] ; de Souza-Chies, T. T. ^[2] ; Souza, F. H. D. ^[3] ; Batista, L. A. R. ^[3] ; Dall'Agnol, M. ^[4] ; Valls, J. F. M. ^[5] ; Zucchi, M. I. ^[6] ; Souza, A. P. ^{[7, 1]}

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, CBMEG, BR-13083970 Campinas, SP - Brazil

^[2] Univ Fed Rio Grande do Sul, Inst Biociencias, Dept Bot, BR-91501970 Porto Alegre, RS - Brazil

^[3] Embrapa Pecuaria Sudeste, BR-13560970 Sao Carlos, SP - Brazil

^[4] Univ Fed Rio Grande do Sul, Fac Agron, Dept Plantas Forrageiras & Agrometeorol, BR-91501970 Porto Alegre, RS - Brazil

^[5] Embrapa Ctr Nacl Recursos Genet CENARGEN, BR-70770900 Brasilia, DF - Brazil

^[6] Apta, Polo Reg Ctr Sul, BR-13400970 Piracicaba, SP - Brazil

^[7] Univ Estadual Campinas, Inst Biol, Dept Biol Vegetal, BR-13083970 Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: AMERICAN JOURNAL OF BOTANY; v. 97, n. 11, p. E107-E110, NOV 2010.
• Citações Web of Science: 8

Resumo

Premise of the study: Paspalum atratum is a perennial, cespitose, tropical grass native to Central and South America. This species belongs to a polyploid complex (Plicatula group) little known at the genetic level. The characterized microsatellite markers provide new informative tools for further studies of the hybridization, mating systems, and structure of the population. Methods and Results: Using the microsatellite-enriched library method, we isolated and characterized 19 microsatellite markers from P. atratum. Eleven of them were polymorphic, showing a variable degree of variation, while eight were monomorphic in the samples analyzed. Additionally, the transferability of these microsatellite markers was tested in other species. Conclusions: These results suggest that the characterized markers have enough discriminatory potential to be used in genetic characterizations of Paspalum taxa, which are based on an understanding of their mating systems and genetic structure, as well as in understanding the evolutionary processes involved in the evolution of groups of Paspalum. (AU)

• Processo FAPESP: 06/61242-8 - Desenvolvimento e caracterização de marcadores moleculares microssatélites em espécies forrageiras nativas
• Beneficiário: Fernanda Witt Cidade
• Linha de fomento: Bolsas no Brasil - Doutorado