Deleção dos genes putative invasin e DnaK de uma linhagem de Escherichia coli patogênica para aves (APEC) para estudar seu efeito na patogenicidade e obtenção de linhagens vacinais

Resumo

Brazil is the third larger producer and the larger exporter of poultry meat world-wide. The production is estimated to be near about 12 millions of ton. Aproximatly 4 millions of tons is exported yearly, by which earning income is about US$ 6 billions. Species of avian pathogenic Escherichia coli (APEC) are agents of significant degrees of morbidity and mortality for poultry and laying hens, causing losses to the poultry industry. A plethora of years, Prof. Wanderley and his colleagues have published works on APEC species for characterizing them with respect to their biological characteristics related to patogenicity (adhesion and invasion of cell species ability, resistance to antimicrobials, expression of fimbriae and flagella), genetics (existence of pathogenicity related genes in plasmids and chromosomes) and many more characteristics. In this proposed work, we aim to study on two important genes, which are known as putative invasin and DnaK, potentially involved in the pathogenicity of an APEC. By deletion of these two genes, we would be able to reduce pathogenicity process in APEC swollen head syndrome strain SCI07 and construct attenuated strains to be used as vaccines against APEC infections as well. (AU)