Crystallisation and protein crystallographic studies of a number of biologically important proteins.

• Processo: 96/02285-5
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Data de Início da vigência: 01 de setembro de 1996
• Data de Término da vigência: 31 de outubro de 1998
• Área do conhecimento: Ciências Biológicas - Bioquímica - Biologia Molecular
• Pesquisador responsável: Igor Polikarpov
• Beneficiário: Igor Polikarpov
• Instituição Sede: Associação Brasileira de Tecnologia de Luz Síncrotron (ABTLuS). Ministério da Ciência, Tecnologia e Inovação (Brasil). Campinas , SP, Brasil
• Bolsa(s) vinculada(s): 97/13891-6 - Crystallisation and protein crystallographic studies of a number of biologically important proteins, BP.TT
• Assunto(s): Radiação síncrotron Proteínas
• Palavra(s)-Chave do Pesquisador: Crystallisation Macromoleculas | Mutant Studies | Protein | Structure Function Relations | Synchrotron Radiation

Resumo

The processes of life depend fundamentally on the interactions and geometrical three-dimensional (3D) structure of the molecules in the living cell. To determine 3D structures the only general method available is X-ray diffraction. The project aims to determine the high resolution crystal structures of several biologically important enzymes such as lysine-ketoglutarate reductase-saccacharopine dehydrogenase, opaque2, glucosamine-6-phosphate synthase, beta-lactoglobulin, desulforedoxin and investigate their structure-function relationships. The proteins will be crystallized using random search method as well as standard techniques of variation of precipitant concentration over a wide range of pH's and their structures will be resolved with use of synchrotron radiation. (AU)