| Processo: | 99/04340-1 |
| Modalidade de apoio: | Auxílio à Pesquisa - Programa GENOMA |
| Data de Início da vigência: | 01 de novembro de 1999 |
| Data de Término da vigência: | 31 de outubro de 2003 |
| Área do conhecimento: | Ciências Biológicas - Genética |
| Pesquisador responsável: | Patricia Brant Monteiro |
| Beneficiário: | Patricia Brant Monteiro |
| Instituição Sede: | Fundo de Defesa da Citricultura (FUNDECITRUS). Araraquara , SP, Brasil |
| Bolsa(s) vinculada(s): | 01/02078-0 - Production and characterization of nonpathogenic mutants of Xylella fastidiosa CVC-strain, the causal agent of the citrus variegated chlorosis (CVC), BP.TT |
| Assunto(s): | Análise de sequência de DNA Clorose variegada dos citros Xylella fastidiosa Genoma Xylella fastidiosa |
| Palavra(s)-Chave do Pesquisador: | Avirulent Genes | Bacterial Transformation | Citrus Variagated Chlorosis | Virulent Geneds | Xylem Limited Pathogens | Xyllela Fastidiosa |
Resumo
Xylella fastidiosa is the casual bacterium of Citrus Variegated Chlorosis (CVC), one of the most severe disease in Brazilian citriculture. With the genome sequencing of CVC X. fastidiosa, putative virulence determinants will be identified, but for their evaluation, it is necessary to develop systems for genetic manipulation of this microorganism. The purpose of our project is to identify genes involved in the bacterial pathogenicity by characterizing nonpathogenic mutants. To produce and characterize X. fastidiosa mutants, gene transfer systems that include a transformation method (electroporation with purified plasmid DNA or conjugation with E. coli donor strains), gene vectors (suicide for gene disruption/replacement and replicative for functional complementation of mutants), and mutagenesis (site-directed by homologous recombination and random by transposon insertion) will be developed for the screening of nonpathogenic mutants. (AU)
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