Human transcript validation initiative

• Processo: 00/12513-2
• Modalidade de apoio: Auxílio à Pesquisa - Programa GENOMA
• Data de Início da vigência: 01 de dezembro de 2000
• Data de Término da vigência: 31 de março de 2003
• Área do conhecimento: Ciências Biológicas - Biologia Geral
• Pesquisador responsável: João Bosco Pesquero
• Beneficiário: João Bosco Pesquero
• Instituição Sede: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brasil
• Assunto(s): Análise de sequência de DNA DNA complementar Etiquetas de sequências expressas

Resumo

Coordination laboratories will be responsible for gene selection and distribution according to the bioinformatics orientation, primer ordering and distribution as well as for providing high quality RNA and first-strand cDNA synthesis. Each Validator Laboratory will have to validate at least 9 full-length genes per month for a two-year period. Groups are required to have experience in RT-PCR, insert subcloning and sequencing as well as equipment and laboratory facilities. There will be no requirement for extensive sequence-finishing and all sequences generated will be submitted to the bioinformatics group as an EST. (AU)