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Human transcript validation initiative

Grant number: 00/12513-2
Support Opportunities:Genome Research Grants
Duration: December 01, 2000 - March 31, 2003
Field of knowledge:Biological Sciences - Biology
Principal Investigator:João Bosco Pesquero
Grantee:João Bosco Pesquero
Host Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil


Coordination laboratories will be responsible for gene selection and distribution according to the bioinformatics orientation, primer ordering and distribution as well as for providing high quality RNA and first-strand cDNA synthesis. Each Validator Laboratory will have to validate at least 9 full-length genes per month for a two-year period. Groups are required to have experience in RT-PCR, insert subcloning and sequencing as well as equipment and laboratory facilities. There will be no requirement for extensive sequence-finishing and all sequences generated will be submitted to the bioinformatics group as an EST. (AU)

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