Human transcript validation initiative

• Grant number: 00/12513-2
• Support Opportunities: Genome Research Grants
• Start date: December 01, 2000
• End date: March 31, 2003
• Field of knowledge: Biological Sciences - Biology
• Principal Investigator: João Bosco Pesquero
• Grantee: João Bosco Pesquero
• Host Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil

Abstract

Coordination laboratories will be responsible for gene selection and distribution according to the bioinformatics orientation, primer ordering and distribution as well as for providing high quality RNA and first-strand cDNA synthesis. Each Validator Laboratory will have to validate at least 9 full-length genes per month for a two-year period. Groups are required to have experience in RT-PCR, insert subcloning and sequencing as well as equipment and laboratory facilities. There will be no requirement for extensive sequence-finishing and all sequences generated will be submitted to the bioinformatics group as an EST. (AU)