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Validator laboratory for the human transcript validation initiative

Grant number: 00/12490-2
Support type:Genome Research Grants
Duration: December 01, 2000 - June 30, 2003
Field of knowledge:Biological Sciences - Biochemistry - Molecular Biology
Principal researcher:Enilza Maria Espreafico
Grantee:Enilza Maria Espreafico
Home Institution: Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

The aim of the project is to validate the structure and to define the sequences for at least 2,000 full-length human genes in a two-year period (216/validator laboratory). Gene sequences will be extracted from genomic sequence available through the Human Genome Project. Sequences will be used as a scaffold for EST (conventional and ORESTES) mapping as well as for gene prediction by computational analysis. Primers for validation of putative genes will be generated based on EST mapping, computational exon prediction and patterns of genomic organization, such a as average gene size, intron/exons size and distribution. Validation will be done mainly by RT-PCR, insert subcloning and sequencing. A databank for gene cataloguing wll be created and also will provide additional information as chromosomal location, expression pattern and alternative transcrits forms. (AU)