Caracterização da resposta imune inata contra vírus em cracas Balanus improvisus (Thecostraca: Thoracica) com ênfase em Vago.

• Processo: 25/01088-6
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado
• Data de Início da vigência: 01 de maio de 2025
• Data de Término da vigência: 30 de abril de 2026
• Área de conhecimento: Ciências Biológicas - Genética - Genética Molecular e de Microorganismos
• Pesquisador responsável: Sónia Cristina da Silva Andrade
• Beneficiário: Rafael Eiji Iwama
• Supervisor: Andreas Hejnol
• Instituição Sede: Instituto de Biociências (IB). Universidade de São Paulo (USP). São Paulo , SP, Brasil
• Instituição Anfitriã: Friedrich Schiller University Jena, Alemanha
• Vinculado à bolsa: 22/14490-9 - Genômica de Coronula diadema e Xenobalanus globicipitis e identificação de genes relacionados à associação ecológica entre cracas e cetáceos, BP.PD
• Assunto(s): Imunidade Caracterização funcional
• Palavra(s)-Chave do Pesquisador: barnacle | Functional Characterization | Immunity | vago | Caracterização Funcional

Resumo

Inhibition of viral replication by host organisms is an essential process of the innate immune response and many pathways are associated with this function in Metazoa. In invertebrates, four main intracellular immune pathways are related to the immunity against viruses: the cyclic GMP-AMP synthase (cGAS) and stimulator of interferon genes (STING), RIG-I-like, Toll-like receptors (TLRs) and RNA interference (RNAi). However, the evolution of these pathways in Metazoa is characterized by several events of gain and losses of key components. An interesting example is the origin of interferon and Vago, two analogous cytokines that are responsible to induce the JAK/STAT pathway. While interferon is restricted to vertebrates, Vago is only found in arthropods. Although Vago has been reported in several lineages of Pancrustacea, it is only functionally characterized in Drosophila melanogaster and the shrimp Litopenaeus vannamei. The present proposal aims to understand the functional origin of Vago in pancrustacea by functionally characterizing Vago in the barnacle model species Amphibalanus improvisus Darwin, 1854. Cultured nauplii and cyprid larvae will be challenged with Poly(I:C), a mimic of viral infection, and expression will be characterized through RNAseq differential expression and in-situ hybridization.