Effect of low-level laser therapy on odontoblast-like cells exposed to bleaching agent

Autor(es)

Lima, Adriano Fonseca ^{[1, 2]} ; Dias Ribeiro, Ana Paula ^[3] ; Basso, Fernanda Goncalves ^[4] ; Bagnato, Vanderlei Salvador ^[5] ; Hebling, Josimeri ^[6] ; Marchi, Giselle Maria ^[1] ; de Souza Costa, Carlos Alberto ^[7]

Afiliação do(s) autor(es)

^[1] Univ Campinas UNICAMP, Piracicaba Dent Sch, Dept Restorat Dent, BR-13404903 Sao Paulo - Brazil

^[2] Nove de Julho Univ, Dept Restorat Dent, BR-01504000 Sao Paulo - Brazil

^[3] Univ Brasilia UnB, Dept Operat Dent, BR-70910900 Brasilia, DF - Brazil

^[4] Univ Campinas UNICAMP, Piracicaba Dent Sch, Dept Pathol, BR-13404903 Sao Paulo - Brazil

^[5] Univ Sao Paulo, Phys Inst Sao Carlos, BR-13560970 Sao Paulo - Brazil

^[6] Univ Estadual Paulista UNESP, Araraquara Sch Dent, Dept Orthodont & Pediat Dent, BR-14801903 Sao Paulo - Brazil

^[7] Univ Estadual Paulista UNESP, Araraquara Sch Dent, Dept Physiol & Pathol, BR-14801903 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Lasers in Medical Science; v. 29, n. 5, p. 1533-1538, SEP 2014.
• Citações Web of Science: 6

Resumo

The aim of the present study was to evaluate the effect of low-level laser therapy (LLLT) on odontoblast-like MDPC-23 cells exposed to carbamide peroxide (CP 0.01 %-2.21 mu g/mL of H2O2). The cells were seeded in sterile 24-well plates for 72 h. Eight groups were established according to the exposure or not to the bleaching agents and the laser energy doses tested (0, 4, 10, and 15 J/cm(2)). After exposing the cells to 0.01 % CP for 1 h, this bleaching solution was replaced by fresh culture medium. The cells were then irradiated (three sections) with a near-infrared diode laser (InGaAsP-780 +/- 3 nm, 40 mW), with intervals of 24 h. The 0.01 % CP solution caused statistically significant reductions in cell metabolism and alkaline phosphate (ALP) activity when compared with those of the groups not exposed to the bleaching agent. The LLLT did not modulate cell metabolism; however, the dose of 4 J/cm(2) increased the ALP activity. It was concluded that 0.01 % CP reduces the MDPC-23 cell metabolism and ALP activity. The LLLT in the parameters tested did not influence the cell metabolism of the cultured cells; nevertheless, the laser dose of 4 J/cm(2) increases the ALP activity in groups both with and without exposure to the bleaching agent. (AU)

• Processo FAPESP: 09/08992-7 - Efeito da aplicação direta e transamelodentinária do laser de baixa potência sobre células odontoblastóides expostas a agentes clareadores
• Beneficiário: Adriano Fonseca de Lima
• Modalidade de apoio: Bolsas no Brasil - Doutorado