| Texto completo | |
| Autor(es): Mostrar menos - |
Lima-Neto, L. G.
[1, 2]
;
Hirata, R. D. C.
[1]
;
Luchessi, A. D.
[1]
;
Silbiger, V. N.
[1]
;
Cavichioli, D.
[1]
;
Dos Santos, E. S.
[3]
;
Sousa, A. G. M.
[3]
;
Sprovieri, S. R. S.
[4]
;
De Sousa-Junior, E. B.
[4]
;
Dos Santos, F. C. P.
[5]
;
Colombo, S.
[5]
;
Hirata, M. H.
[1]
Número total de Autores: 12
|
| Afiliação do(s) autor(es): | [1] Univ Sao Paulo, Sch Pharmaceut Sci, Sao Paulo - Brazil
[2] CEUMA Univ, Immunol Lab, BR-65075120 Sao Luis, MA - Brazil
[3] Inst Dante Pazzanese Cardiol, Sao Paulo - Brazil
[4] Santa Casa Sao Paulo, Sch Med Sci, Sao Paulo - Brazil
[5] Adolfo Lutz Inst, Sao Paulo - Brazil
Número total de Afiliações: 5
|
| Tipo de documento: | Artigo Científico |
| Fonte: | JOURNAL OF BIOLOGICAL REGULATORS AND HOMEOSTATIC AGENTS; v. 28, n. 3, p. 449-460, JUL-SEP 2014. |
| Citações Web of Science: | 4 |
| Resumo | |
We investigated the relationship of the positivity for Chlamydophila pneumoniae (Cpn) and Mycoplasma pneumonia (Mpn), inflammatory and metabolic markers, and mRNA expression and polymorphisms of the TLR2, TLR4, IL-6 and TNFA genes with acute myocardial infarction (AMI). Two hundred and eighteen individuals (98 AMI and 120 non-AMI) were selected at two Clinical Centers. Blood samples were drawn to extract DNA and RNA and to measure laboratory variables including anti-Cpn IgM and IgG. Cpn and Mpn genomic DNA as well as TLR2, TLR4, IL-6 and TNFA mRNA expression were evaluated by quantitative real-time PCR (qPCR). Gene polymorphisms were detected by PCR-HRM. AMI patients had higher positivity for Cpn-DNA (17.3%) than non-AMI group (6.7%, p=0.018). In addition, Cpn-DNA positivity was an independent predictor of risk for AMI (OR: 2.56, CI: 1.08 - 6.04, p=0.031). Positivity for anti-Cpn IgG and Mpn-DNA was similar between AMI and non-AMI (p>0.05). TLR4 mRNA expression was higher in AMI than non-AMI individuals (p=0.005). CD14 -260C>T, TNFA -308A>G, TLR2 c.2258G>A, TLR4 c.896A>G and TLR4 c.1196C>T variants were not associated with increased risk for AMI (p>0.05). In the AMI group, individuals carrying CD14 -260CC genotype had higher hsCRP levels than CT/TT carriers (p=0.041). These results are suggestive that Cpn-DNA positivity and increased TLR4 mRNA expression in blood leukocytes may be associated with AMI and could be useful markers to evaluate the severity and progression of the atherosclerotic disease in AMI patients. (AU) | |
| Processo FAPESP: | 06/04163-8 - Participação da Chlamydia pneumoniae e Mycoplasma pneumoniae no infarto agudo do miocárdio: possível mecanismo via TLR |
| Beneficiário: | Mario Hiroyuki Hirata |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |