Apoptosis in HEp-2 cells infected with Ureaplasma diversum

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Amorim, Aline Teixeira ^[1] ; Marques, Lucas Miranda ^[2] ; Oliveira Gusmao Santos, Angelita Maria ^[3] ; Martins, Hellen Braga ^[4] ; Barbosa, Maysa Santos ^[5] ; Rezende, Izadora Souza ^[6] ; Andrade, Ewerton Ferraz ^[7] ; Campos, Guilherme Barreto ^[8] ; Lobao, Tassia Neves ^[9] ; Cortez, Beatriz Araujo ^[10] ; Monezi, Telma Alvez ^[11] ; Machado-Santelli, Glaucia Maria ^[12] ; Timenetsky, Jorge ^[13]

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^[1] Univ Fed Bahia. Inst Multidisciplinar Saude

^[2] Univ Fed Bahia. Inst Multidisciplinar Saude

^[3] Univ Sao Paulo. Inst Ciencias Biomed

^[4] Univ Fed Bahia. Inst Multidisciplinar Saude

^[5] Univ Fed Bahia. Inst Multidisciplinar Saude

^[6] Univ Fed Bahia. Inst Multidisciplinar Saude

^[7] Univ Fed Bahia. Inst Multidisciplinar Saude

^[8] Univ Sao Paulo. Inst Ciencias Biomed

^[9] Univ Sao Paulo. Inst Ciencias Biomed

^[10] Univ Sao Paulo. Inst Ciencias Biomed

^[11] Univ Sao Paulo. Inst Ciencias Biomed

^[12] Univ Sao Paulo. Inst Ciencias Biomed

^[13] Univ Sao Paulo. Inst Ciencias Biomed

• Tipo de documento: Artigo Científico
• Fonte: Biological Research; v. 47, SEP 4 2014.
• Citações Web of Science: 0

Resumo

BACKGROUND: Bacterial pathogens have many strategies for infecting and persisting in host cells. Adhesion, invasion and intracellular life are important features in the biology of mollicutes. The intracellular location ofUreaplasma diversum may trigger disturbances in the host cell. This includes activation or inhibition of pro and anti-apoptotic factors, which facilitate the development of host damage. The aim of the present study was to associate U. diversum infection in HEp-2 cells and apoptosis induction. Cells were infected for 72hs with four U. diversum clinical isolates and an ATCC strain. The U. diversuminvasion was analyzed by Confocal Laser Scanning Microscopy and gentamicin invasion assay. The apoptosis was evaluated using pro-apoptotic and anti-apoptotic gene expression, and FITC Annexin V/Dead Cell Apoptosis Kit. RESULTS: The number of internalized ureaplasma in HEp-2 cells increased significantly throughout the infection. The flow cytometry analysis with fluorochromes to detect membrane depolarization and gene expression for caspase 2, 3 and 9 increased in infected cells after 24 hours. However, after 72 hours a considerable decrease of apoptotic cells was observed. CONCLUSIONS: The data suggests that apoptosis may be initially induced by some isolates in association with HEp-2 cells, but over time, there was no evidence of apoptosis in the presence of ureaplasma and HEp-2 cells. The initial increase and then decrease in apoptosis could be related to bacterial pathogen-associated molecular pattern (PAMPS). Moreover, the isolates of U. diversum presented differences in the studied parameters for apoptosis. It was also observed that the amount of microorganisms was not proportional to the induction of apoptosis in HEp-2 cells. (AU)

• Processo FAPESP: 12/00719-2 - Estudo dos fatores de virulência de ureaplasmas bovinos isolados no semi-árido da Bahia
• Beneficiário: Jorge Timenetsky
• Modalidade de apoio: Auxílio à Pesquisa - Regular