Application of laser ablation (imaging) inductively coupled plasma mass spectrometry for mapping and quantifying Fe in transgenic and non-transgenic soybean leaves

Autor(es)

Oliveira, Silvana R. ^[1] ; Arruda, Marco A. Z. ^[2]

Afiliação do(s) autor(es)

^[1] Univ Campinas Unicamp, Natl Inst Sci & Technol Bioanalyt INCTBio, Inst Chem, Dept Analyt Chem, BR-13083970 Campinas, SP - Brazil

^[2] Univ Campinas Unicamp, Spectrometry Sample Preparat & Mechanizat Grp GEP, BR-13083970 Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Analytical Atomic Spectrometry; v. 30, n. 2, p. 389-395, 2015.
• Citações Web of Science: 15

Resumo

Laser ablation inductively coupled plasma mass spectrometry is used for determining Fe in leaves of transgenic (variety M 7211RR) and non-transgenic (variety MSOY 8200) soybean, grown for 21 days in a growth chamber at controlled temperature (27 +/- 0.1 degrees C) and photoperiod (12 h). The same technique, but in the imaging mode, is also employed for acquiring the spatial distribution of Fe in leaves of both the varieties. For attaining both proposals, different parameters, such as RF power, carrier and auxiliary gas flow rates, dynamic reaction cell flow rate, RPq, laser intensity, spot diameter, frequency and scan speed, are optimized. The accuracy of the laser ablation method is assessed by comparing the results (from ratios between Fe-56 and C-13, used as an internal standard) obtained from pellet samples (200 mg of the certified reference materials-NIST SRM 1515 and 1573a at a pressure of 7 psi), or prepared pellets from transgenic and non-transgenic soybean leaves, with those from digested samples. No differences at 95% confidence levels are found. Although Fe concentrations are similar in transgenic and non-transgenic leaves, their spatial distribution is significantly different. Fe is homogenously distributed in the whole transgenic soybean leaf, while this element is greatly concentrated in the main vein and nerves of the non-transgenic leaf. Taking into account all the variables that are controlled during the experiment, this result indicates that genetic modification might be responsible for altering the Fe distribution in the soybean leaves. (AU)

• Processo FAPESP: 11/12054-2 - Avaliação da distribuição isotópica de 57Fe em diferentes partes de plantas de soja (Glicine max L. Merril) transgênica e não-transgênica empregando ICP-MS e mapeamento por meio de imagens empregando LA-ICP-MS
• Beneficiário: Silvana Ruella de Oliveira
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado