Microsatellite in Aeschynomene falcata (Leguminosae): diversity, cross-amplification, and chromosome localization

Autor(es)

Polido, C. A. ^{[1, 2]} ; Mantello, C. C. ^[3] ; Moraes, A. P. ^{[4, 1]} ; Souza, A. P. ^{[3, 1]} ; Forni-Martins, E. R. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Inst Biol, Dept Biol Vegetal, Programa Posgrad Biol Vegetal, Campinas, SP - Brazil

^[2] Escolas & Fac MAGSUL, Ponta Pora, MS - Brazil

^[3] Univ Estadual Campinas, Ctr Biol Mol & Engn Genet, Campinas, SP - Brazil

^[4] Univ Estadual Paulista, Inst Biociencias, Dept Genet, Botucatu, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Genetics and Molecular Research; v. 13, n. 4, p. 10390-10397, 2014.
• Citações Web of Science: 0

Resumo

Aeschynomene falcata is an important forage species; however, because of low seed production, it is underutilized as forage species. Aeschynomene is a polyphyletic genus with a challenging taxonomic position. Two subgenera have been proposed, and it is suggested that Aeschynomene can be split in 2 genera. Thus, new markers, such as microsatellite sequences, are desirable for improving breeding programs for Aeschynomene falcata. Based on transferability and in situ localization, these microsatellite sequences can be applied as chromosome markers in the genus Aeschynomene and closely related genera. Here, we report the first microsatellite library developed for this genus; 11 microsatellites were characterized, with observed and expected heterozygosities ranging from 0.0000 to 0.7143 and from 0.1287 to 0.8360, respectively. Polymorphic information content varied from 0.1167 to 0.7786. The departure from Hardy-Weinberg equilibrium may have resulted from frequent autogamy, which is characteristic of Aeschynomene falcata. Of the 11 microsatellites, 9 loci were cross-amplified in Aeschynomene brevipes and Aeschynomene paniculata and 7 in Dalbergia nigra and Machaerium vestitum. Five of these 7 cross-amplified microsatellites were applied as probes during the in situ hybridization assay and 2 showed clear signals on Aeschynomene falcata chromosomes, ensuring their viability as chromosome markers. (AU)

• Processo FAPESP: 11/22215-3 - Respostas do genoma vegetal às variações ambientais: o que as Orchidaceae têm a contar?
• Beneficiário: Ana Paula de Moraes
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 10/19132-6 - Estudo cromossômico na tribo Dalbergieae sensu Klitgaard & Lavin (2005), com ênfase no clado "informal" Dalbergia (Leguminosae, Papilionoideae) e na aplicação de técnicas moleculares para a obtenção de novas sondas para a hibridação de DNA in situ
• Beneficiário: Eliana Regina Forni Martins
• Modalidade de apoio: Auxílio à Pesquisa - Regular