Improvement of gamete quality and its short-term storage: an approach for biotechnology in laboratory fish

Autor(es)

Yasui, G. S. ^[1] ; Senhorini, J. A. ^[2] ; Shimoda, E. ^[3] ; Pereira-Santos, M. ^[4] ; Nakaghi, L. S. O. ^[4] ; Fujimoto, T. ^[5] ; Arias-Rodriguez, L. ^[6] ; Silva, L. A. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Vet Med FZEA, Lab Theriogenol Dr OJ Ginther, BR-13630080 Pirassununga, SP - Brazil

^[2] Natl Ctr Res & Conservat Continental Fish, Chico Mendes Inst Biodivers Conservat, BR-13630970 Pirassununga, SP - Brazil

^[3] Univ Candido Mendes, Dept Pharm, BR-28030335 Campos Dos Goytacazes, RJ - Brazil

^[4] Sao Paulo State Univ, Aquaculture Ctr, BR-14884900 Jaboticabal, SP - Brazil

^[5] Hokkaido Univ, Fac Fisheries Sci, Hakodate, Hokkaido 0418611 - Japan

^[6] Juarez Autonomous Univ Tabasco, Biol Sci Acad Div, Villahermosa 86150, Tabasco - Mexico

• Tipo de documento: Artigo Científico
• Fonte: ANIMAL; v. 9, n. 3, p. 464-470, MAR 2015.
• Citações Web of Science: 19

Resumo

In fish, in vitro fertilization is an important reproductive tool used as first step for application of others biotechniques as chromosome and embryo manipulation. In this study, we aimed to optimize gamete quality and their short-term storage from the yellowtail tetra Astyanax altiparanae, for future application in laboratory studies. Working with sperm, we evaluated the effects of spawning inducers (carp pituitary gland and Ovopel (R) {[}(D-Ala6, Pro9-NEt) - mGnRH+metoclopramide]) and the presence of female on sperm motility. Additionally, we developed new procedures for short-term storage of sperm and oocytes. Briefly, sperm motility was higher when male fish were treated with carp pituitary gland (73.1 +/- 4.0%) or Ovopel (R) (79.5 +/- 5.5%) when compared with the control group treated with 0.9% NaCl (55.6 +/- 27.2%; P=0.1598). Maintenance of male fish with an ovulating female fish also improved sperm motility (74.4 +/- 7.4%) when compared with untreated male fish (42.1 +/- 26.1%; P=0.0018). Storage of sperm was optimized in modified Ringer solution, in which the sperm was kept motile for 18 days at 2.5 degrees C. The addition of antibiotics or oxygen decreased sperm motility, but partial change of supernatant and the combination of those conditions improve storage ability of sperm. Fertilization ability of oocytes decreased significantly after storage for 30, 60 90 and 120 min at 5, 10, 15 and 20 degrees C when compared with fresh oocytes (P=0.0471), but considering only the stored samples, the optimum temperature was 15 degrees C. Those data describe new approaches to improve semen quality and gametes short-term storage in yellowtail tetra A. altiparanae and open new possibilities in vitro fertilization. (AU)

• Processo FAPESP: 10/17429-1 - Quimerismo em espécies nativas de peixes como ferramenta para a propagação mediada na aquicultura
• Beneficiário: George Shigueki Yasui
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 11/11664-1 - Quimerismo em espécies nativas de peixes como ferramenta para a propagação mediada na aquicultura
• Beneficiário: George Shigueki Yasui
• Modalidade de apoio: Bolsas no Brasil - Jovens Pesquisadores