AMH and AMHR2 Polymorphisms and AMH Serum Level Can Predict Assisted Reproduction Outcomes: A Cross-Sectional Study

Autor(es)

Peluso, Carla ^[1] ; Fonseca, Fernando L. A. ^[2] ; Gastaldo, Guilherme G. ^[1] ; Christofolini, Denise M. ^[1] ; Cordts, Emerson Barchi ^[1] ; Barbosa, Caio P. ^[1] ; Bianco, Bianca ^[1]

Afiliação do(s) autor(es)

^[1] Fac Med ABC, Dept Collect Hlth, Human Reprod & Genet Ctr, BR-09060650 Santo Andre, SP - Brazil

^[2] Fac Med ABC, Dept Clin Med, Lab Clin Anal, BR-09060650 Santo Andre, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: CELLULAR PHYSIOLOGY AND BIOCHEMISTRY; v. 35, n. 4, p. 1401-1412, 2015.
• Citações Web of Science: 13

Resumo

Background: In human assisted reproduction, the ovarian response to exogenous recombinant Follicle-stimulating Hormone (FSH) therapy is variable and difficult to predict. The standard protocol of ovarian hyperstimulation can result in satisfactory response; however, an unsatisfactory response necessitates FSH dose adjustment or results in ovarian hyperstimulation syndrome (OHSS). Polymorphisms in AMH and AMHR2 genes appear to affect hormone biological activities, thus affecting follicle recruitment and development, leading to infertility. We aimed to evaluate AMH and AMHR2 polymorphisms in infertile women, and correlate those findings with AMH, FSH and estradiol serum level response to controlled ovarian hyperstimulation (COH), as well as assisted reproduction outcomes. Methods: A cross-sectional study comprising 186 infertile women that underwent one cycle of high complexity assisted reproductive treatment. Blood samples were collected and a TaqMan assay was used for AMH G146T/rs10407022 and AMHR2 A-482G/rs2002555, A10G/rs11170555, C1749G/rs2071558 and G4952A/rs3741664 genotyping, and FSH, estradiol and AMH levels were measured. The findings were correlated to human reproduction outcomes. Results: AMH rs10407022 and AMHR2 rs2002555 polymorphisms were not associated with hormonal measurements, whereas AMHR2 rs11170555 and rs3741664 were positively associated with AMH, estradiol and FSH levels. The genotype distribution of AMH and AMHR2 genes according to Controlled Ovarian Hyperstimulation did not show a positive association. However, an association with AFC, degree of oocyte maturation (allele G of AMHR2 rs2071558) the number of embryos produced (alleles T and G of AMH rs10407022 and AMHR2 rs2002555, respectively) and frozen embryo (allele G of AMHR2 rs11170555) were found to be statistically associated. Considering COH, serum AMH and AFC were a positive predictor to OHSS. Regarding serum AMH and assisted reproduction outcomes, a positive correlation with all variables studied was found. Comparing AFC and AMH as predictors of human reproduction outcomes, the AFC was less effective than serum AMH. Considering pregnancy rates, no marker was positively associated. Conclusion: AMHR2 polymorphisms were associated with estradiol, AMH and FSH measurements, as well as number and quality of embryos, while AMH polymorphisms was associated with number of embryos produced. Serum AMH was correlated with nearly all variables analyzed in assisted reproductive treatment, demonstrating that it represents a better biomarker of OHSS and human reproduction outcomes compared to AMH and AMHR2 polymorphisms. Copyright (C) 2015 S. Karger AG, Basel (AU)

• Processo FAPESP: 13/06989-4 - Polimorfismos do gene do receptor do hormônio anti-müleriano em mulheres inférteis e sua correlação com a estimulação ovariana controlada
• Beneficiário: Guilherme George Gastaldo
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 11/08681-1 - Avaliação de polimorfismos do gene FSHr, Amh e AMHR2 em mulheres inférteis e sua correlação com resultados de reprodução assistida
• Beneficiário: Bianca Alves Vieira Bianco
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 11/15045-4 - Avaliação de polimorfismos dos genes Amh e AMHR2 em mulheres inférteis e sua correlação com resultados de reprodução assistida
• Beneficiário: Carla Peluso de Paiva
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 14/06177-2 - Avaliação de mutações/polimorfismos em genes candidatos em mulheres inférteis e sua correlação com resultados de reprodução assistida
• Beneficiário: Bianca Alves Vieira Bianco
• Modalidade de apoio: Auxílio à Pesquisa - Regular