Immunofluorescence assay for diagnosis of strongyloidiasis in immunocompromised patients

Autor(es)

Gottardi, Maiara ^[1] ; Paula, Fabiana M. ^[1] ; Corral, Marcelo A. ^[1] ; Meisel, Dirce Mary C. ^[1] ; Costa, Silvia F. ^[2] ; Abdala, Edson ^[2] ; Pierrotti, Ligia C. ^[3] ; Yamashiro, Juliana ^[3] ; Chieffi, Pedro Paulo ^[4] ; Gryschek, Ronaldo Cesar B. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Hosp Clin, Lab Invest Med LIM 06, Fac Med, BR-05403000 Sao Paulo, SP - Brazil

^[2] Univ Sao Paulo, Fac Med, Dept Molestias Infecciosas & Parasitarias, BR-05403000 Sao Paulo, SP - Brazil

^[3] Univ Sao Paulo, Fac Med, Hosp Clin, BR-05403000 Sao Paulo, SP - Brazil

^[4] Santa Casa Sao Paulo Sch Med Sci, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: INFECTIOUS DISEASES; v. 47, n. 8, p. 550-554, AUG 2015.
• Citações Web of Science: 1

Resumo

Background: Strongyloides stercoralis is a parasite that causes human strongyloidiasis. The disease ranges from asymptomatic to severe forms, which are often fatal in immunocompromised individuals. Laboratory diagnosis is challenging owing to limitations in the use of conventional parasitological techniques. The present study aimed to evaluate the indirect immunofluorescence assay (IFA) using infective larvae of S. venezuelensis as an antigen for the immunodiagnosis of human strongyloidiasis in immunocompromised patients. Methods: Serum and stool samples from 200 immunocompromised patients (HIV-positive, HTLV-1-positive, and renal, liver, and/or bone marrow transplantation candidates) were used. Stool samples were examined using three parasitological methods: Lutz, Rugai, and culture agar plate. IFA was performed using sections of infective larvae of S. venezuelensis as antigens, and showed 95.4% sensitivity and 95.8% and specificity. Results: Among the 200 patients, 17 (8.5%) were positive for S. stercoralis by at least one parasitological method, and 43 (21.5%) were positive by IFA. Conclusions: IFA can be used as a screening method for the detection of S. stercoralis in immunocompromised patients. (AU)

• Processo FAPESP: 10/51110-2 - Investigação de técnicas diagnósticas para identificação da infecção por S. stercoralis em pacientes com diferentes formas de imunodepressão
• Beneficiário: Pedro Paulo Chieffi
• Modalidade de apoio: Auxílio à Pesquisa - Regular