Analysis of iron superoxide dismutase-encoding DNA vaccine on the evolution of the Leishmania amazonensis experimental infection

Autor(es)

Campos, B. L. S. ^[1] ; Silva, T. N. ^[1] ; Ribeiro, S. P. ^{[2, 3]} ; Carvalho, K. I. L. ^[2] ; Kallas, E. G. ^{[2, 3]} ; Laurenti, M. D. ^[1] ; Passero, L. F. D. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Med, Lab Pathol Infect Dis LIM50, BR-01246000 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Sch Med, Lab Clin Immunol & Allergy LIM60, BR-01246000 Sao Paulo - Brazil

^[3] Inst Invest Immunol Iii INCT, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PARASITE IMMUNOLOGY; v. 37, n. 8, p. 407-416, AUG 2015.
• Citações Web of Science: 14

Resumo

The present work aimed to evaluate the immunogenicity of Leishmania amazonensis iron superoxide dismutase (SOD) -encoding DNA experimental vaccine and the protective properties of this DNA vaccine during infection. The SOD gene was subcloned into the pVAX1 plasmid, and it was used to immunize BALB/c mice. Twenty-one days after the last immunization, mice were sacrificed (immunogenicity studies) or subcutaneously challenged with L. amazonensis (studies of protection), and alterations in cellular and humoral immune responses were evaluated, as well as the course of infection. Mice only immunized with pVAX1-SOD presented increased frequencies of CD4(+) IFN-gamma(+), CD8(+) IFN- gamma(+) and CD8(+) IL-4(+) lymphocytes; moreover, high levels of IgG2a were detected. After challenge, mice that were immunized with pVAX1-SOD had increased frequencies of the CD4(+) IL- 4(+), CD8(+) IFN-gamma(+) and CD8(+) IL-4(+) T lymphocytes. In addition, the lymph node cells produced high amounts of IFN-gamma and IL-4 cytokines. Increased IgG2a was also detected. The pattern of immunity induced by pVAX1-SOD partially protected the BALB/c mice from a challenge with L. amazonensis, as the animals presented reduced parasitism and lesion size when compared to controls. Taken together, these results indicate that leishmanial SOD modulates the lymphocyte response, and that the elevation in IFN-gamma possibly accounted for the decreased skin parasitism observed in immunized animals. (AU)

• Processo FAPESP: 12/10409-0 - Estudo das propriedades imunogênicas e protetoras de vacinas de terceira geração contra a leishmaniose tegumentar americana
• Beneficiário: Luiz Felipe Domingues Passero
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 12/05219-8 - Análise de imunogenicidade e proteção gerada pelas vacinas de terceira geração pVAX1-FSD e pVAX1-SP contra a Leishmaniose Tegumentar Americana experimental
• Beneficiário: Bruno Luiz Soares Campos
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 11/51157-1 - Vacinas de DNA contra leishmaniose tegumentar americana
• Beneficiário: Luiz Felipe Domingues Passero
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado