Neo-sex chromosomes of Ronderosia bergi: insight into the evolution of sex chromosomes in grasshoppers

Autor(es)

Palacios-Gimenez, O. M. ^[1] ; Marti, D. A. ^[2] ; Cabral-de-Mello, D. C. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista, UNESP, Dept Biol, Inst Biociencias, BR-13506900 Rio Claro, SP - Brazil

^[2] Univ Nacl Misiones, Fac Ciencias Exactas Quim & Nat, Lab Genet Evolut, IBS, CONICET, RA-3300 Posadas - Argentina

• Tipo de documento: Artigo Científico
• Fonte: CHROMOSOMA; v. 124, n. 3, p. 353-365, SEP 2015.
• Citações Web of Science: 13

Resumo

Sex chromosomes have evolved many times from morphologically identical autosome pairs, most often presenting several recombination suppression events, followed by accumulation of repetitive DNA sequences. In Orthoptera, most species have an X0a (TM), sex chromosome system. However, in the subfamily Melanoplinae, derived variants of neo-sex chromosomes (neo-XYa (TM), or neo-X1X2Ya (TM),) emerged several times. Here, we examined the differentiation of neo-sex chromosomes in a Melanoplinae species with a neo-XYa (TM),/XXa (TM) Euro system, Ronderosia bergi, using several approaches: (i) classical cytogenetic analysis, (ii) mapping via fluorescent in situ hybridization of some selected repetitive DNA sequences and microdissected sex chromosomes, and (iii) immunolocalization of distinct histone modifications. The microdissected sex chromosomes were also used as sources for Polymerase chain reaction (PCR) amplification of RNA-coding multigene families, to study variants related to the sex chromosomes. Our data suggest that the R. bergi neo-Y has become differentiated after its formation by a Robertsonian translocation and inversions, and has accumulated repetitive DNA sequences. Interestingly, the ex autosomes incorporated into the neo-sex chromosomes retain some autosomal post-translational histone modifications, at least in metaphase I, suggesting that the establishment of functional modifications in neo-sex chromosomes is slower than their sequence differentiation. (AU)

• Processo FAPESP: 12/01421-7 - Contribuições a elucidação da estrutura, origem e evolução de neo-cromossomos em gafanhotos utilizando como modelo espécies dos gêneros Chlorus, Dichromatos e Eurotettix (Melanoplinae; Acrididae)
• Beneficiário: Octavio Manuel Palacios Gimenez
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 14/11763-8 - Contribuições ao entendimento da estrutura e evolução de sistemas de cromossomos sexuais utilizando como modelos espécies de gafanhotos e grilos
• Beneficiário: Diogo Cavalcanti Cabral de Mello
• Modalidade de apoio: Auxílio à Pesquisa - Regular