The Cerato-Platanin protein Epl-1 from Trichoderma harzianum is involved in mycoparasitism, plant resistance induction and self cell wall protection

Autor(es):Mostrar menos -

Gomes, Eriston Vieira ^[1] ; Costa, Mariana do Nascimento ^[1] ; de Paula, Renato Graciano ^[1] ; de Azevedo, Rafael Ricci ^[2] ; da Silva, Francilene Lopes ^[3] ; Noronha, Eliane F. ^[3] ; Ulhoa, Cirano Jose ^[4] ; Monteiro, Valdirene Neves ^[5] ; Cardoza, Rosa Elena ^[6] ; Gutierrez, Santiago ^[6] ; Silva, Roberto Nascimento ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Biochem & Immunol, BR-14049 Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Mol & Cellular Biol & Pathogen Bioagents, BR-14049 Ribeirao Preto, SP - Brazil

^[3] Univ Brasilia, Dept Cellular Biol, Brasilia, DF - Brazil

^[4] Univ Fed Goias, Inst Biol Sci, Dept Biochem & Cellular Biol, Goiania, Go - Brazil

^[5] State Univ Goias, Dept Biochem, Anapolis, Go - Brazil

^[6] Univ Leon, Univ Sch Agr Engineers, Dept Microbiol, Ponferrada - Spain

• Tipo de documento: Artigo Científico
• Fonte: SCIENTIFIC REPORTS; v. 5, DEC 9 2015.
• Citações Web of Science: 22

Resumo

Trichoderma harzianum species are well known as biocontrol agents against important fungal phytopathogens. Mycoparasitism is one of the strategies used by this fungus in the biocontrol process. In this work, we analyzed the effect of Epl-1 protein, previously described as plant resistance elicitor, in expression modulation of T. harzianum genes involved in mycoparasitism process against phytopathogenic fungi; self cell wall protection and recognition; host hyphae coiling and triggering expression of defense-related genes in beans plants. The results indicated that the absence of Epl-1 protein affects the expression of all mycoparasitism genes analyzed in direct confrontation assays against phytopathogen Sclerotinia sclerotiorum as well as T. harzianum itself; the host mycoparasitic coiling process and expression modulation of plant defense genes showing different pattern compared with wild type strain. These data indicated the involvement T. harzianum Epl-1 in self and host interaction and also recognition of T. harzianum as a symbiotic fungus by the bean plants. (AU)

• Processo FAPESP: 12/16895-4 - Estudo de genes de Trichoderma harzianum associados ao micoparasitismo e seu papel no biocontrole de Sclerotinia sclerotiorum
• Beneficiário: Eriston Vieira Gomes
• Modalidade de apoio: Bolsas no Brasil - Doutorado