Hyaluronan/chitosan nanofilms assembled layer-by-layer and their antibacterial effect: A study using Staphylococcus aureus and Pseudomonas aeruginosa

Autor(es)

Hernandez-Montelongo, J. ^[1] ; Lucchesi, E. G. ^[2] ; Gonzalez, I. ^[3] ; Macedo, W. A. A. ^[3] ; Nascimento, V. F. ^[4] ; Moraes, A. M. ^[4] ; Beppu, M. M. ^[4] ; Cotta, M. A. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Inst Fis Gleb Wataghin, Dept Fis Aplicada, BR-13083859 Campinas, SP - Brazil

^[2] Itibanyl Prod Especiais LTDA, BR-13240000 Jarinu, SP - Brazil

^[3] Ctr Desenvolvimento Tecnol Nucl, BR-31270901 Belo Horizonte, MG - Brazil

^[4] Univ Estadual Campinas, Fac Engn Quim, BR-13083852 Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: COLLOIDS AND SURFACES B-BIOINTERFACES; v. 141, p. 499-506, MAY 1 2016.
• Citações Web of Science: 21

Resumo

In the last few years, chitosan-based coatings have been proposed as antibacterial surfaces for biomedical devices in order to prevent nosocomial infections. In that sense, this work reports the optimized synthesis of hyaluronan/chitosan (HA/CHI) nanofilms assembled layer-by-layer in order to maximize the antibacterial effect for two important human pathogenic bacteria, Staphylococcus aureus and Pseudomonas aeruginosa. In this assembly, HA forms a soft, highly hydrated, and nontoxic film, whereas CHI shows the antimicrobial characteristics. Our HA/CHI nanofilm synthesis optimization was based on changing pH values of the biopolymer stem-solutions and the consequent variation of their ionization degree. Furthermore, the surface density of primary amino groups, which are related to the antibacterial effect, was also enhanced by increasing the number of HA/CHI bilayers. The antibacterial effect of HA/CHI nanofilms was evaluated by the spread plate counting method for both bacteria. These results were correlated with the morphology of nanofilms (characterized using SEM and AFM), as well as with their chemical properties studied by UV-vis, Kelvin Probe Force microscopy and XPS spectroscopy. (C) 2016 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 13/02300-1 - Nanofios semicondutores: mecanismos de formação e aplicação em biossensores
• Beneficiário: Mônica Alonso Cotta
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 10/51748-7 - Análise estrutural e química de biofilmes de Xylella fastidiosa
• Beneficiário: Mônica Alonso Cotta
• Modalidade de apoio: Auxílio à Pesquisa - Regular