Cytokine profile and lymphocyte subsets in type 2 diabetes

Autor(es)

Francisco, C. O. ^[1] ; Catai, A. M. ^[1] ; Moura-Tonello, S. C. G. ^[1] ; Arruda, L. C. M. ^{[2, 3]} ; Lopes, S. L. B. ^[4] ; Benze, B. G. ^[5] ; Del Vale, A. M. ^[4] ; Malmegrim, K. C. R. ^[6] ; Leal, A. M. O. ^[4]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Carlos, Dept Fisioterapia, BR-13560 Sao Carlos, SP - Brazil

^[2] Univ Sao Paulo, Dept Bioquim & Imunol, Fac Med Ribeirao Preto, BR-14049 Ribeirao Preto, SP - Brazil

^[3] Fundacao Amparo Pesquisa Estado Sao Paulo, Ctr Terapia Celular, Ribeirao Preto, SP - Brazil

^[4] Univ Fed Sao Carlos, Dept Med, BR-13560 Sao Carlos, SP - Brazil

^[5] Univ Fed Sao Carlos, Dept Estat, BR-13560 Sao Carlos, SP - Brazil

^[6] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Dept Anal Clin Toxicol & Bromatol, BR-14049 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Brazilian Journal of Medical and Biological Research; v. 49, n. 4 2016.
• Citações Web of Science: 5

Resumo

Type 2 diabetes mellitus (T2D) is a metabolic disease with inflammation as an important pathogenic background. However, the pattern of immune cell subsets and the cytokine profile associated with development of T2D are unclear. The objective of this study was to evaluate different components of the immune system in T2D patients' peripheral blood by quantifying the frequency of lymphocyte subsets and intracellular pro- and anti-inflammatory cytokine production by T cells. Clinical data and blood samples were collected from 22 men (51.6±6.3 years old) with T2D and 20 nonsmoking men (49.4±7.6 years old) who were matched for age and sex as control subjects. Glycated hemoglobin, high-sensitivity C-reactive protein concentrations, and the lipid profile were measured by a commercially available automated system. Frequencies of lymphocyte subsets in peripheral blood and intracellular production of interleukin (IL)-4, IL-10, IL-17, tumor necrosis factor-α, and interferon-γ cytokines by CD3+ T cells were assessed by flow cytometry. No differences were observed in the frequency of CD19+ B cells, CD3+CD8+ and CD3+CD4+ T cells, CD16+56+ NK cells, and CD4+CD25+Foxp3+ T regulatory cells in patients with T2D compared with controls. The numbers of IL-10- and IL-17-producing CD3+ T cells were significantly higher in patients with T2D than in controls (P<0.05). The frequency of interferon-γ-producing CD3+ T cells was positively correlated with body mass index (r=0.59; P=0.01). In conclusion, this study shows increased numbers of circulating IL-10- and IL-17-producing CD3+ T cells in patients with T2D, suggesting that these cytokines are involved in the immune pathology of this disease. (AU)

• Processo FAPESP: 11/51007-0 - Avaliacao da funcao cardiopulmonar e sua interrelacao com mediadores inflamatorios no diabetes mellitus.
• Beneficiário: Ângela Merice de Oliveira Leal
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 10/12437-6 - Avaliação das inter-relações da função cardiopulmonar e mediadores inflamatórios no diabetes mellitus
• Beneficiário: Cristina de Oliveira Francisco
• Modalidade de apoio: Bolsas no Brasil - Mestrado