Low-level laser therapy in 3D cell culture model using gingival fibroblasts

Autor(es)

Basso, Fernanda G. ^[1] ; Soares, Diana G. ^[1] ; de Souza Costa, Carlos Alberto ^[1] ; Hebling, Josimeri ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista UNESP, Araraquara Sch Dent, R Humaita 1680, BR-14801903 Araraquara, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Lasers in Medical Science; v. 31, n. 5, p. 973-978, JUL 2016.
• Citações Web of Science: 6

Resumo

Besides extensive data about the effects of low-level laser therapy (LLLT) on different cell types, so far, these results were obtained from monolayer cell culture models, which have limitations in terms of cell morphology and phenotype expression. Therefore, for better in vitro evaluation of the effects of LLLT, this study was performed with a 3D cell culture model, where gingival fibroblasts were seeded in collagen matrix. Cells isolated from a healthy patient were seeded in wells of 24-well plates with culture medium (DMEM) supplemented with 10 % fetal bovine serum and collagen type I solution. After 5 days, a serum-free DMEM was added to the matrices with cells that were subjected or not to three consecutive irradiations of LLLT by means of the LaserTABLE diode device (780 nm, 25 mW) at 0.5, 1.5, and 3 J/cm(2). Twenty-four hours after the last irradiation, cell viability and morphology as well as gene expression of growth factors were assessed. Histological evaluation of matrices demonstrated uniform distribution and morphology of gingival fibroblasts within the collagen matrix. LLLT at 3 J/cm(2) increased gingival fibroblast viability. Enhanced gene expression of hCOL-I and hEGF was observed for 0.5 J/cm(2), while no significant changes were detected for the other irradiation densities tested. In conclusion, LLLT promoted biostimulation of gingival fibroblasts seeded in a 3D cell culture model, demonstrating that this model can be applied for phototherapy studies and that LLLT could penetrate the collagen matrix to increase cell functions related to tissue repair. (AU)

• Processo FAPESP: 13/05879-0 - Efeito da laserterapia de baixa potência e fatores de crescimento sobre a atividade de fibroblastos de gengiva obtidos de pacientes jovens e idosos
• Beneficiário: Carlos Alberto de Souza Costa
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 12/17947-8 - Efeito do laser de baixa intensidade sobre a expressão de citocinas inflamatórias na mucosite oral
• Beneficiário: Fernanda Gonçalves Basso
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado