Mitogen activated protein kinases SakA(HOG1) and MpkC collaborate for Aspergillus fumigatus virulence

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de Oliveira Bruder Nascimento, Ariane Cristina Mendes ^[1] ; dos Reis, Thaila Fernanda ^[1] ; de Castro, Patricia Alves ^[1] ; Hori, Juliana I. ^[1] ; Pedro Bom, Vinicius Leite ^[1] ; de Assis, Leandro Jose ^[1] ; Zambelli Ramalho, Leandra Naira ^[2] ; Rocha, Marina Campos ^[3] ; Malavazi, Iran ^[3] ; Brown, Neil Andrew ^[4] ; Valiante, Vito ^[5] ; Brakhage, Axel A. ^{[6, 7]} ; Hagiwara, Daisuke ^[8] ; Goldman, Gustavo H. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Ribeirao Preto - Brazil

^[2] Univ Sao Paulo, Fac Med Ribeirao Preto, Ribeirao Preto - Brazil

^[3] Univ Fed Sao Carlos, Ctr Ciencias Biol & Saude, Dept Genet & Evolucao, Sao Carlos, SP - Brazil

^[4] Rothamsted Res, Plant Sci & Crop Biol, Harpenden, Herts - England

^[5] Leibniz Inst Nat Prod Res & Infect Biol, Hans Knoll Inst, Leibniz Jr Res Grp Biobricks Microbial Nat Prod S, Jena - Germany

^[6] Leibniz Inst Nat Prod Res & Infect Biol HKI, Dept Mol & Appl Microbiol, Jena - Germany

^[7] Univ Jena, Inst Microbiol, Jena - Germany

^[8] Chiba Univ, Med Mycol Res Ctr, Chiba - Japan

• Tipo de documento: Artigo Científico
• Fonte: Molecular Microbiology; v. 100, n. 5, p. 841-859, JUN 2016.
• Citações Web of Science: 26
• Assunto(s): Parede celular Saccharomyces cerevisiae Candida albicans

Resumo

Here, we investigated which stress responses were influenced by the MpkC and SakA mitogen-activated protein kinases of the high-osmolarity glycerol (HOG) pathway in the fungal pathogen Aspergillus fumigatus. The DsakA and the double Delta mpkC Delta sakA mutants were more sensitive to osmotic and oxidative stresses, and to cell wall damaging agents. Both MpkC:: GFP and SakA:: GFP translocated to the nucleus upon osmotic stress and cell wall damage, with SakA:: GFP showing a quicker response. The phosphorylation state of MpkA was determined post exposure to high concentrations of congo red and Sorbitol. In the wild-type strain, MpkA phosphorylation levels progressively increased in both treatments. In contrast, the Delta sakA mutant had reduced MpkA phosphorylation, and surprisingly, the double Delta mpkC Delta sakA had no detectable MpkA phosphorylation. A. fumigatus DsakA and DmpkC were virulent in mouse survival experiments, but they had a 40% reduction in fungal burden. In contrast, the Delta mpkC Delta sakA double mutant showed highly attenuated virulence, with approximately 50% mice surviving and a 75% reduction in fungal burden. We propose that both cell wall integrity (CWI) and HOG pathways collaborate, and that MpkC could act by modulating SakA activity upon exposure to several types of stresses and during CW biosynthesis. (AU)

• Processo FAPESP: 14/00789-6 - Caracterização funcional de fosfatases de Aspergillus nidulans envolvidas no metabolismo da glicose
• Beneficiário: Leandro Jose de Assis
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado