Gingival crevicular fluid levels of protease-activated receptors type 1 and type 2 in diabetic patients with periodontitis

Autor(es)

Abreu, I. S. ; Euzebio Alves, V. T. ; Benedete, A. P. S. ; Bueno da Silva, H. A. ; Franca, B. N. ; Saraiva, L. ; Lima, L. A. ; Carvalho, M. H. ; Holzhausen, M.

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF PERIODONTAL RESEARCH; v. 51, n. 5, p. 577-585, OCT 2016.
• Citações Web of Science: 0

Resumo

Background and Objective: Protease activated receptor type 1 (PAR1) seems to play a role in periodontal repair, while PAR2 is associated with periodontal inflammation. As diabetes is a known risk factor for periodontal disease, the aim of this study was to evaluate the influence of type 2 diabetes on PAR1 and PAR2 mRNA expression in the gingival crevicular fluid of patients with chronic periodontitis before and after non-surgical periodontal treatment. Material and Methods: Gingival crevicular fluid samples and clinical parameters consisting of measuring probing depth, clinical attachment level, bleeding on probing and plaque index were collected from systemically healthy patients and patients with type 2 diabetes and chronic periodontitis, at baseline and after non-surgical periodontal therapy. PAR1 and PAR2, as well as the presence of the proteases RgpB gingipain and neutrophil proteinase-3 were assessed by quantitative polymerase chain reaction in the gingival crevicular fluid. Results: The periodontal clinical parameters significantly improved after periodontal therapy (p < 0.01). Diabetes led to increased expression of PAR1 in gingival crevicular fluid, and in the presence of chronic periodontitis, it significantly decreased the expression of PAR1 and PAR2 (p < 0.05). Moreover, non-surgical periodontal treatment in diabetics resulted in increased expression of PAR1 and PAR2 (p < 0.05), and decreased expression of RgpB gingipain and proteinase-3 (p < 0.05). Conclusion: The present data demonstrated that diabetes was associated with an altered expression of PAR1 and PAR2 in the gingival crevicular fluid cells of subjects with chronic periodontitis. Future studies are necessary to elucidate the effects of PAR1 upregulation in periodontally healthy sites and PAR2 downregulation in chronic periodontitis sites on the increased susceptibility and severity of periodontitis in diabetes. (AU)

• Processo FAPESP: 09/16373-5 - Avaliação de biomarcadores de estresse oxidativo em pacientes com Diabetes Mellitus tipo 2 com periodonto saudável e com periodontite crônica generalizada, antes e após tratamento periodontal
• Beneficiário: Luiz Antonio Pugliesi Alves de Lima
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 10/20477-8 - Avaliação da expressão do receptor ativado por protease do tipo 2 (PAR-2) em pacientes com periodontite crônica, antes e após tratamento periodontal não-cirúrgico.
• Beneficiário: Vanessa Tubero Euzebio Alves
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 14/06387-7 - Expressão do receptor ativado por protease tipo 2 (PAR-2) em células do fluido gengival de pacientes diabéticos do tipo 2
• Beneficiário: Bruno Nunes de França
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 10/16605-0 - Efeito da terapia periodontal sobre a expressão de receptor ativado por protease do tipo 2 (PAR-2) em pacientes com periodontite crônica.
• Beneficiário: Marinella Holzhausen Caldeira
• Modalidade de apoio: Auxílio à Pesquisa - Regular