Apoptosis-associated protein expression in human salivary gland morphogenesis

Autor(es)

Teshima, Tathyane H. N. ; Ianez, Renata C. F. ; Coutinho-Camillo, Claudia M. ; Tucker, Abigail S. ; Lourenco, Silvia V.

• Tipo de documento: Artigo Científico
• Fonte: ARCHIVES OF ORAL BIOLOGY; v. 69, p. 71-81, SEP 2016.
• Citações Web of Science: 7

Resumo

Objective: Salivary gland (SG) development is based on branching morphogenesis, in which programmed cell death has been proposed to play a role in cell signalling and organ shaping. In the mouse salivary gland apoptosis has been suggested to play a key role in lumen formation, removing the central cells of the epithelial stalks. Here we analyse the expression of several anti- and pro-regulators of apoptosis during human SG development in a range of developmental stages. Design: Foetal SGs obtained from the University of Sao Paulo were analysed by immunohistochemistry to assess the expression of apoptosis-associated proteins: caspases (caspase-6, -7, -9 and cleaved caspase-3), Bcl-2 family members (Bax, Bak, Bad, Bid, Bcl-2, Bcl-x and Bcl-xL), Survivin (BIRC5), Cytochrome C and Apaf-1. Results: Nuclear expression of Bax and Bak was identified in presumptive luminal areas at initial stages, while Bcl-xL showed the most relevant anti-apoptotic activity. Caspase-6, -7 and -9 were expressed during all stages, while interestingly cleaved caspase-3 showed no prominent expression, indicating that caspase-7 is the main effector. Apoptosome complex components Apaf-1 and Cytochrome C, as well as survivin were all positive in developing glands. Conclusions: The particular expression pattern of several apoptotic regulators in human SG development suggests the existence of a fundamental role for apoptosis during duct formation. The absence of Bad and Bid expressions indicates that the instrinsic pathway is more active then the extrinsic during human gland formation. The subcellular localisation of intrinsic-apoptosis proteins correlated with apoptotic activity, but also suggested additional non-apoptotic functions. (C) 2016 Published by Elsevier Ltd. (AU)

• Processo FAPESP: 15/02824-6 - Desenvolvimento e regeneração de glândulas salivares: análise de linhagem condicional transgênica sob controle específico temporal e tecidual
• Beneficiário: Tathyane Harumi Nakajima Teshima
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado Direto
• Processo FAPESP: 11/18865-2 - Investigação da atividade apoptótica na abertura luminal dos ductos das glândulas salivares: análise comparativa entre modelo animal e humano
• Beneficiário: Tathyane Harumi Nakajima Teshima
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto