Effects of TGF-beta 1 on mineralization mediated by rat calvaria-derived osteogenic cells

Autor(es)

Donato, Tatiani A. G. ; Martinez, Elizabeth F. ; Arana-Chavez, Victor E.

• Tipo de documento: Artigo Científico
• Fonte: MICROSCOPY RESEARCH AND TECHNIQUE; v. 79, n. 12, p. 1139-1146, DEC 2016.
• Citações Web of Science: 1

Resumo

In this study, we have analyzed the viability and cell growth, as well as, the mineralization of extracellular matrix (ECM) by alizarin red and von Kossa staining of calvaria-derived osteogenic cultures, treated with TGF-beta 1 alone or associated with Dex comparing with acid ascorbic (AA) + beta-glicero-phosphate (bGP) (positive mineralization control). The expression of the noncollagenous proteins bone sialoprotein (beta SP), osteopontin (OPN) and fibronectin (FN) were evaluated by indirect immunofluorescence. In addition, the main ultrastructural morphological findings were assessed by transmission electron microscopy. Osteogenic cells were isolated of calvaria bone from newborn (2-day-old) Wistar rats were treated with TGF-beta 1 alone or with dexamethasone for 7, 10, and 14 days. As positive mineralization control, the cells were supplemented only with AA1 beta GP. As negative control, the cells were cultured with basal medium (alpha-MEM + 10%FBS + 1%gentamicin). The treatment with TGF-beta 1, even when combined with Dex, decreased the viability and cell growth when compared with the positive control. Osteoblastic cell cultures were positive to alizarin red and von Kossa stainings after AA + beta GP and Dex alone treatments. Positive immunoreaction was found for BSP, OPN and FN in all studied treatments. Otherwise, when the cell cultures were supplemented with TGF-beta 1 and TGF-beta 1 + Dex, no mineralization was observed in any of the studied periods. These present findings suggest that TGF-beta 1, in the studied in vitro doses, inhibits the proliferation and differentiation of osteoblastic cells by impairment of nodule formation. (AU)

• Processo FAPESP: 10/18094-3 - Efeito do fator de transformação de crescimento -beta 1 (TGF-²1) e da dexametasona na biomineralização: estudo in vitro nas vias de sinalização
• Beneficiário: Victor Elias Arana-Chavez
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 06/50153-4 - Estudo de proteínas não-colágenas da matriz em cultura de células pulpares humanas induzidas com fatores mineralizantes através de imunocitoquímica, imunofluorescência e Western-blotting
• Beneficiário: Victor Elias Arana-Chavez
• Modalidade de apoio: Auxílio à Pesquisa - Regular