Room temperature fluorescence spectroscopy of benzo[a]pyrene metabolites on octadecyl extraction membranes

Autor(es)

Alfarhani, Bassam ; Al-tameemi, Maha ; Schenone, Agustina V. ; Goicoechea, Hector C. ; Barbosa, Jr., Fernando ; Campiglia, Andres D.

• Tipo de documento: Artigo Científico
• Fonte: Microchemical Journal; v. 129, p. 83-89, NOV 2016.
• Citações Web of Science: 1

Resumo

Benzo{[}a]pyrene (B{[}a]P) is a prototypic carcinogenic polycyclic aromatic hydrocarbon (PAH), which requires metabolic activation to produce its detrimental effects. Measurement of B{[}a]P metabolites in human urine could provide a direct way to assess individual differences in susceptibility to PAH-related cancer. This article focuses on the development of screening methodology for the routine analysis of B{[}a]P metabolites in urine samples. It explores the solid-surface room-temperature fluorescence (RTF) properties of 3-hydroxybenzo{[}a]pyrene, benzo{[}a]pyrene-trans-9,10-dihydrodiol, benzo{[}a]pyrene-r-7,t-8,c-9-tetrahydrotriol and benzo{[}a]pyrene-r-7,t-8,c-9,c-10-tetrahydrotetrol previously extracted from urine samples with octadecyl-silica membranes. Relative standard deviations varying from 2.1% (benzo{[}a]pyrene-r-7,t-8,c-9-tetrahydrotriol) to 8.6% (3-hydroxy-benzo{[}a]pyrene) are obtained with the aid of fiber optic probe that eliminates the need for manual optimization of signal intensities. Analytical recoveries from human urine samples varied from 87.5 +/- 3.1% (3-hydroxy-benzo{[}a]pyrene) to 99.8 +/- 2.5% (benzo{[}a]pyrene-r-7,t-8,c-9,c-10-tetrahydrotetrol). The excellent analytical figures of merit and the simplicity of the experimental procedure demonstrate the potential of this approach for screening biomarkers of PAH exposure in numerous urine samples. (C) 2016 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 14/00986-6 - Desenvolvimento de um novo método para monitoramento em rotina de metabólitos de hidrocarbonetos policíclico aromáticos (HPAs) em amostras de urina
• Beneficiário: Fernando Barbosa Júnior
• Modalidade de apoio: Auxílio à Pesquisa - Pesquisador Visitante - Internacional