Ultrasound as a safe and reliable guidance for subarachnoid puncture in rabbits

Autor(es)

Ivan Dias Fernandes Pereira ^[1] ; Roberto Bezerra Vital ^[2] ; Ronaldo Silva ^[3] ; Felipe Gilberto Valerini ^[4] ; Vania Maria de Vasconcelos Machado ^[5] ; Mariangela Esrher Marques ^[6] ; Helio Miot ^[7] ; Lais Helena Navarro ^[8] ; Eliana Marisa Ganem ^[9]

Afiliação do(s) autor(es)

^[1] Universidade Estadual Paulista. Department of Anesthesiology - Brasil

^[2] UNESP. Department of Anesthesiology. Postgraduate Program in Anesthesiology - Brasil

^[3] UNESP. Department of Anesthesiology. Postgraduate Program in Anesthesiology - Brasil

^[4] UNESP. Botucatu Medical School - Brasil

^[5] UNESP. Department of Animal Reproduction and Veterinary Radiology - Brasil

^[6] UNESP. Department of Pathology - Brasil

^[7] UNESP. Department of Dermatology - Brasil

^[8] UNESP. Department of Anesthesiology - Brasil

^[9] UNESP. Department of Anesthesiology - Brasil

• Tipo de documento: Artigo Científico
• Fonte: Acta Cirurgica Brasileira; v. 32, n. 1, p. 14-21, 2017-01-00.

Resumo

Abstract Purpose: To evaluate a model for studying the toxicity in nervous tissue and meninges using ultrasound to guide needle insertion into the subarachnoid space of rabbits, with the objective of avoiding injuries triggered by the puncture and by intraneural injection of solutions. Methods: Forty-five adult female rabbits were divided into 3 groups (G): G1 underwent subarachnoid puncture, G2 underwent subarachnoid injection of saline solution and G3 underwent subarachnoid injection of 0.5% hyperbaric bupivacaine. A needle was inserted into the S1-S2 subarachnoid space guided by ultrasound. The sensitivity and motility of the animals were evaluated for 3 days, after which the animals were sacrificed for removal of lumbar and sacral portions of the spinal cord for histological examination by light microscopy and immunohistochemistry. Results: All animals had motor function and pain sensitivity on the evaluation period. No animal had complication during or after the procedures. Conclusion: Ultrasound is a useful and safe method for the correct identification of the subarachnoid space of rabbits. (AU)

• Processo FAPESP: 09/54490-3 - Modelo experimental para estudo de toxidade do tecido nervoso e de meninges em coelhos utilizando ulta-som para orientar puncao subaracnoidea.
• Beneficiário: Eliana Marisa Ganem
• Modalidade de apoio: Auxílio à Pesquisa - Regular