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DIAGNOSIS OF Strongyloides stercoralis INFECTION IN IMMUNOCOMPROMISED PATIENTS BY SEROLOGICAL AND MOLECULAR METHODS

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de Paula, Fabiana Martins ; Malta, Fernanda Mello ; Corral, Marcelo Andreetta ; Marques, Priscilla Duarte ; Gottardi, Maiara ; Correia Lima Meisel, Dirce Mary ; Yamashiro, Juliana ; Rebello Pinho, Joao Renato ; Pagliusi Castilho, Vera Lucia ; do Nascimento Goncalves, Elenice Messias ; Borges Gryschek, Ronaldo Cesar ; Chieffi, Pedro Paulo
Número total de Autores: 12
Tipo de documento: Artigo Científico
Fonte: Revista do Instituto de Medicina Tropical de São Paulo; v. 58, 2016.
Citações Web of Science: 7
Resumo

Strongyloidiasis is a potentially serious infection in immunocompromised patients. Thus, the availability of sensitive and specific diagnostic methods is desirable, especially in the context of immunosuppressed patients in whom the diagnosis and treatment of strongyloidiasis is of utmost importance. In this study, serological and molecular tools were used to diagnose Strongyloides stercoralis infections in immunosuppressed patients. Serum and stool samples were obtained from 52 patients. Stool samples were first analyzed by Lutz, Rugai, and Agar plate culture methods, and then by a quantitative real time polymerase chain reaction (qPCR). Serum samples were evaluated by an enzyme-linked immunosorbent assay (ELISA) using a soluble (AS) or a membrane fractions antigen (AM) obtained from alkaline solutions of the filariform larvae of Strongyloides venezuelensis. Of the 52 immunosuppressed patients, three (5.8%) were positive for S. stercoralis by parasitological methods, compared to two patients (3.8%) and one patient (1.9%) who were detected by ELISA using the AS and the AM antigens, respectively. S. stercoralis DNA was amplified in seven (13.5%) stool samples by qPCR. These results suggest the utility of qPCR as an alternative diagnostic tool for the diagnosis of S. stercoralis infection in immunocompromised patients, considering the possible severity of this helminthiasis in this group of patients. (AU)

Processo FAPESP: 10/51110-2 - Investigação de técnicas diagnósticas para identificação da infecção por S. stercoralis em pacientes com diferentes formas de imunodepressão
Beneficiário:Pedro Paulo Chieffi
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 13/04236-9 - Imunoproteômica aplicada ao aprimoramento do diagnóstico da estrongiloidíase humana frente a diferentes extratos antigênicos de Strongyloides venezuelensis
Beneficiário:Ronaldo Cesar Borges Gryschek
Linha de fomento: Auxílio à Pesquisa - Regular