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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Effects of oocytes exposure to bovine diarrhea viruses BVDV-1, BVDV-2 and Hobi-like virus on in vitro-produced bovine embryo development and viral infection

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da Silva Cardoso Pinto, V. ; Alves, M. F. ; de Souza Nunes Martins, M. ; Basso, A. C. ; Tannura, J. H. ; Pontes, J. H. F. ; Santos Lima, M. ; Garcia da Silva, T. ; Okuda, L. H. ; Stefano, E. ; Romaldini, A. H. C. N. ; Arnold, D. R. ; Pituco, E. M.
Número total de Autores: 13
Tipo de documento: Artigo Científico
Fonte: Theriogenology; v. 97, p. 67-72, JUL 15 2017.
Citações Web of Science: 2
Resumo

As production of in vitro (IVP) bovine embryos steadily increases, the sanitary risk associated with IVP embryos remains a concern. One of the greatest concerns is how BVDV may be transmitted through IVP embryos. The objective of this study was to evaluate the effects caused by BVDV-1, BVDV-2 and Hobi-like virus exposure during in vitro maturation on embryo development and viral infection. Abittior-derived oocytes were randomly assigned for in vitro maturation with serial concentrations of BVDV-1 (3.12 x 10(2) - 2.50 x 10(3) TCID50/100 mu L), BVDV-2 (6.25 x 10(1) - 5.20 x 10(2) TCID50/100 mu L) or Hobi-like virus (1.90 x 10(2) - 1.58 x 10(3) TCID50/100 mu L) for 22-24 h. After maturation, oocytes were fertilized and embryo cultured following standard in vitro procedures. Embryo development was evaluated and percentage of respective, positive BVDV degenerated and viable embryos were evaluated by RT-qPCR. No concentration of BVDV-1 altered embryo development as measured by cleavage and blastocyst rates, compared to negative control group. However 100% of degenerated embryos and 50-100% of viable embryos tested positive for BVDV-1, depending on the viral concentration. BVDV-2 exposed oocytes had higher cleavage rates than the negative control group (60.2-64.1% vs 49.8%; P = 0.003-0.032). However, no difference was detected for blastocyst rates. In aadition, 100% of degenerated embryos and 20-50% of viable embryos tested positive for BVDV-2. Hobi-like virus treated oocytes had reduced cleavage rates for the three highest viral concentrations (333-38.0% vs 49.8% for negative controls; P <= 0.001-0.014). Blastocyst rates were only reduced in the 7.9 x 10(2) Hobi-like virus concentration (6.9 +/- 0.9% vs 15.1 +/- 1.6%; P = 0.009), when calculated by oocyte number. 50-80% of degenerated embryos tested positive for Hobi-like virus. No viable embryos from the Hobi-like virus treated oocytes tested positive. These results suggest that IVP embryos from BVDV-1 and -2 infected oocytes develop normally, but carry the virus. However, Hobi-like virus infected oocytes had reduced cleavage and cause pre-implantation embryo loss, but viable embryos did not carry the virus. (C) 2017 Elsevier Inc. All rights reserved. (AU)

Processo FAPESP: 14/50169-4 - Padronização e validação de PCR em tempo real para certificação sanitária de embriões de bovinos produzidos in vitro
Beneficiário:Juliana Hayashi Tannura
Modalidade de apoio: Auxílio à Pesquisa - Pesquisa Inovativa em Pequenas Empresas - PIPE