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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Spatial genetic structure and dispersal of the cacao pathogen Moniliophthora perniciosa in the Brazilian Amazon

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Autor(es):
Artero, A. S. ; Silva, J. Q. ; Albuquerque, P. S. B. ; Bressan, E. A. ; Leal, Jr., G. A. ; Sebbenn, A. M. ; Griffith, G. W. ; Figueira, A.
Número total de Autores: 8
Tipo de documento: Artigo Científico
Fonte: PLANT PATHOLOGY; v. 66, n. 6, p. 912-923, AUG 2017.
Citações Web of Science: 2
Resumo

Moniliophthora perniciosa is the causal agent of witches' broom in Theobroma cacao (cacao). Three biotypes of M.perniciosa are recognized, differing in host specificity, with two causing symptoms on cacao or Solanaceae species (C- and S-biotypes), and the third found growing endophytically on lianas (L-biotype). The objectives of this study were to clarify the genetic relationship between the three biotypes, and to identify those regions in the Brazilian Amazon with the greatest genetic diversity for the C-biotype. Phylogenetic reconstruction based on the rRNA ITS regions showed that the C- and S-biotypes formed a well-supported clade separated from the L-biotype. Analysis of 131 isolates genotyped at 11 microsatellite loci found that S- and especially L-biotypes showed a higher genetic diversity. A significant spatial genetic structure was detected for the C-biotype populations in Amazonia for up to 137km, suggesting isolation by distance' mode of dispersal. However, in regions containing extensive cacao plantings, C-biotype populations were essentially clonal', as evidenced by high frequency of repeated multilocus genotypes. Among the Amazonian C-biotype populations, Acre and West Amazon displayed the largest genotypic diversity and might be part of the centre of diversity of the fungus. The pathogen dispersal may have followed the direction of river flow downstream from Acre, Rondonia and West Amazon eastward to the rest of the Amazon valley, where cacao is not endemic. The Bahia population exhibited the lowest genotypic diversity, but high allele richness, suggesting multiple invasions, with origin assigned to Rondonia and West Amazon, possibly through isolates from the Lower Amazon population. (AU)

Processo FAPESP: 07/07175-0 - Análise genética da resposta de defesa de Theobroma cacao a Moniliophthora perniciosa, agente causal da vassoura-de-bruxa
Beneficiário:Antonio Vargas de Oliveira Figueira
Modalidade de apoio: Auxílio à Pesquisa - Temático
Processo FAPESP: 03/11483-0 - Identificação de regiões genômicas e genes diferencialmente expressos associados à resistência do cacaueiro (Theobroma cacao L.) à vassoura-de-bruxa (agente causal Crinipellis perniciosa)
Beneficiário:Antonio Vargas de Oliveira Figueira
Modalidade de apoio: Auxílio à Pesquisa - Regular