microRNA159-targeted SIGAMYB transcription factors are required for fruit set in tomato

Autor(es)

da Silva, Eder Marques ; Ferreira e Silva, Geraldo Felipe ; Bidoia, Debora Brussolo ; Azevedo, Mariana da Silva ; de Jesus, Frederico Almeida ; Pino, Lilian Ellen ; Pereira Peres, Lazaro Eustaquio ; Carrera, Esther ; Lopez-Diaz, Isabel ; Silveira Nogueira, Fabio Tebaldi

• Tipo de documento: Artigo Científico
• Fonte: Plant Journal; v. 92, n. 1, p. 95-109, OCT 2017.
• Citações Web of Science: 8

Resumo

The transition from flowering to fruit production, namely fruit set, is crucial to ensure successful sexual plant reproduction. Although studies have described the importance of hormones (i.e. auxin and gibberellins) in controlling fruit set after pollination and fertilization, the role of microRNA-based regulation during ovary development and fruit set is still poorly understood. Here we show that the microRNA159/GAMYB1 and -2 pathway (the miR159/GAMYB1/2 module) is crucial for tomato ovule development and fruit set. MiR159 and SlGAMYBs were expressed in preanthesis ovaries, mainly in meristematic tissues, including developing ovules. SlMIR159-overexpressing tomato cv. Micro-Tom plants exhibited precocious fruit initiation and obligatory parthenocarpy, without modifying fruit shape. Histological analysis showed abnormal ovule development in such plants, which led to the formation of seedless fruits. SlGAMYB1/2 silencing in SlMIR159-overexpressing plants resulted in misregulation of pathways associated with ovule and female gametophyte development and auxin signalling, including AINTEGUMENTA-like genes and the miR167/SlARF8a module. Similarly to SlMIR159-overexpressing plants, SlGAMYB1 was downregulated in ovaries of parthenocarpic mutants with altered responses to gibberellins and auxin. SlGAMYBs likely contribute to fruit initiation by modulating auxin and gibberellin responses, rather than their levels, during ovule and ovary development. Altogether, our results unveil a novel function for the miR159-targeted SlGAMYBs in regulating an agronomically important trait, namely fruit set. (AU)

• Processo FAPESP: 15/17892-7 - Estudo funcional de vias reguladas pelos microRNAs miR159 e miR319 durante o desenvolvimento de flores e frutos de tomateiro
• Beneficiário: Fabio Tebaldi Silveira Nogueira
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/02469-6 - Avaliação da contribuição da via de regulação do gene SlyGAMYB1 pelo microRNA159 no desenvolvimento de órgãos laterais e formação de frutos em tomateiro Micro-Tom (Solanum lycopersicum)
• Beneficiário: Eder Marques da Silva
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 15/14286-9 - Superexpressão do SlyMIR159 altera do desenvolvimento floral e promove formação de frutos partenocárpicos em tomateiro
• Beneficiário: Eder Marques da Silva
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado