Determination of Selenium in Bovine Semen by ICP-MS Using Formic Acid for Sample Preparation

Autor(es)

Bianchi, Silmara R. ^{[1, 2]} ; Amaral, Clarice D. B. ^[3] ; Silva, Caroline S. ^{[2, 4]} ; Nogueira, Ana Rita A. ^[2]

Afiliação do(s) autor(es)

^[1] Embrapa Solos, Rua Jardim Bot, 1024 Jardim Bot, BR-22460000 Rio De Janeiro, RJ - Brazil

^[2] Embrapa Pecuaria Sudeste, POB 339, BR-13560970 Sao Carlos, SP - Brazil

^[3] Univ Fed Parana, Dept Quim, POB 19032, BR-81531980 Curitiba, PR - Brazil

^[4] Univ Fed Sao Carlos, Dept Quim, Grp Anal Instrumental Aplicada, POB 676, BR-13560970 Sao Carlos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of the Brazilian Chemical Society; v. 28, n. 12, p. 2357-2364, DEC 2017.
• Citações Web of Science: 2

Resumo

Determination of Se by inductively coupled plasma mass spectrometry (ICP-MS) can be difficult due to spectral interferences; however, these interferences can be eliminated by using collision-reaction interface (CRI) technology. A simple and fast procedure for determination of total Se concentration in bovine semen by ICP-MS after sample solubilization with diluted formic acid is here proposed. Formic acid caused an increase of up to 50% in selenium net signals and the maximum gain was obtained at 10% v v-1 formic acid solution. The optimized nebulizer gas flow rates were 1.05 and 0.95 L min-1, and radio frequency applied power were 1.3 and 1.4 kW without and with CRI, respectively. Microwave-assisted acid digestion was also implemented in the preparation of semen sample for comparison of results. No statistical differences between these sample preparation strategies at 95% confidence level (t-test) were observed for 76Se+, 77Se+, 78Se+ and 82Se+ without using CRI and 82Se+ with CRI. (AU)

• Processo FAPESP: 06/59083-9 - Espectrometria de massa acoplada ao plasma induzido: aspectos fundamentais e aplicações
• Beneficiário: Joaquim de Araújo Nóbrega
• Modalidade de apoio: Auxílio à Pesquisa - Temático