| Texto completo | |
| Autor(es): |
Bertanha, Matheus
[1]
;
Sobreira, Marcone Lima
[1]
;
de Carvalho Bovolato, Ana Livia
[2]
;
Rinaldi, Jaqueline de Carvalho
[3]
;
Reis, Patricia Pintor
[1]
;
Moroz, Andrei
[4]
;
de Moraes, Leonardo Nazario
[5]
;
Deffune, Elenice
[6]
Número total de Autores: 8
|
| Afiliação do(s) autor(es): | [1] Univ Estadual Paulista UNESP, Dept Surg & Orthoped, Botucatu Med Sch, Botucatu, SP - Brazil
[2] UNESP, Botucatu Med Sch, Cell Engn Lab, Postgrad Program Res & Dev Med Biotechnol, Botucatu, SP - Brazil
[3] UNESP, Botucatu Biosci Inst, Botucatu, SP - Brazil
[4] UNESP, Sch Pharmaceut Sci, Dept Bioproc & Biotechnol, Araraquara, SP - Brazil
[5] UNESP, Botucatu Biosci Inst, Postgrad Program Biol Sci Genet, Botucatu, SP - Brazil
[6] UNESP, Botucatu Med Sch, Dept Urol, Botucatu, SP - Brazil
Número total de Afiliações: 6
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Acta Cirurgica Brasileira; v. 32, n. 9, p. 706-711, SEP 2017. |
| Citações Web of Science: | 0 |
| Resumo | |
Abstract Purpose: To investigate the ultrastructural characteristics and analysis of residual DNA in scaffold models, produced with decellularized vena cava in an experimental model with rabbits. Methods: Three groups were created for ultrastructural and residual DNA analysis: group 1 - control, consisting of samples of vena cava in natura; group 2 - SD, consisting of vein fragments submitted to 2% sodium deoxycholate decellularization by shaking (160rpm - Shaker News Brunswick Scientific®) for 1 hour at controlled temperature shaker at 37°C; group 3 - SDS, consisting of vein fragments submitted to 1% sodium dodecyl sulfate decellularization under the same previous condition, for 2 hours. Results: The ultrastructural matrix of the blood vessel maintained its vintegrity after either decellularization models. The results of the two quantification methods demonstrated a significant decrease in the DNA content of the decellularized vena cava samples as compared to the control samples and, differed statistically from each other, p <0.05. Conclusion: The 2% DS protocol for vein decellularization, in this experimental model, was considered the best protocol because it presented less amount of residual DNA without causing substantial destruction of the extracellular matrix. (AU) | |
| Processo FAPESP: | 10/52549-8 - Estruturação ex-vivo de vasos sanguíneos a partir da diferenciação de células tronco de coelhos |
| Beneficiário: | Elenice Deffune |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |