Biotecnologia aplicada ao melhoramento genético de gramíneas para a produção de bi...
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| Texto completo | |
| Autor(es): Mostrar menos - |
de Souza, Wagner R.
[1]
;
Martins, Polyana K.
[1]
;
Freeman, Jackie
[2]
;
Pellny, Till K.
[2]
;
Michaelson, Louise V.
[2]
;
Sampaio, Bruno L.
[1]
;
Vinecky, Felipe
[1]
;
Ribeiro, Ana P.
[1]
;
da Cunha, Barbara A. D. B.
[1]
;
Kobayashi, Adilson K.
[1]
;
de Oliveira, Patricia A.
[1]
;
Campanha, Raquel B.
[1]
;
Pacheco, Thalyta F.
[1]
;
Martarello, Danielly C. I.
[3]
;
Marchiosi, Rogerio
[3]
;
Ferrarese-Filho, Osvaldo
[3]
;
dos Santos, Wanderley D.
[3]
;
Tramontina, Robson
[4]
;
Squina, Fabio M.
[5]
;
Centeno, Danilo C.
[6]
;
Gaspar, Marilia
[7]
;
Braga, Marcia R.
[7]
;
Tine, Marco A. S.
[7]
;
Ralph, John
[8, 9]
;
Mitchell, Rowan A. C.
[2]
;
Molinari, Hugo B. C.
[1]
Número total de Autores: 26
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| Afiliação do(s) autor(es): | [1] EMBRAPA Agroenergy, BR-70770901 Brasilia, DF - Brazil
[2] Rothamsted Res, Plant Sci, Harpenden AL5 2JQ, Herts - England
[3] State Univ Maring, Dept Biochem, BR-87020900 Maringa, Parana - Brazil
[4] Brazilian Ctr Res Energy & Mat, Brazilian Bioethanol Sci & Technol Lab, BR-13083100 Campinas, SP - Brazil
[5] Univ Sorocaba UNISO, Programa Proc Tecnol & Ambientais, BR-18060000 Sorocaba - Brazil
[6] Fed Univ ABC, Ctr Nat Sci & Humanities, BR-09606045 Sao Bernardo Do Campo, SP - Brazil
[7] Inst Bot, Dept Plant Physiol & Biochem, BR-04301012 Sao Paulo - Brazil
[8] Univ Wisconsin, Dept Biochem, 420 Henry Mall, Madison, WI 53705 - USA
[9] Univ Wisconsin, Wisconsin Energy Inst, Dept Energys, Great Lakes Bioenergy Res Ctr, Madison, WI - USA
Número total de Afiliações: 9
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| Tipo de documento: | Artigo Científico |
| Fonte: | NEW PHYTOLOGIST; v. 218, n. 1, p. 81-93, APR 2018. |
| Citações Web of Science: | 19 |
| Resumo | |
Feruloylation of arabinoxylan (AX) in grass cell walls is a key determinant of recalcitrance to enzyme attack, making it a target for improvement of grass crops, and of interest in grass evolution. Definitive evidence on the genes responsible is lacking so we studied a candidate gene that we identified within the BAHD acyl-CoA transferase family. We used RNA interference (RNAi) silencing of orthologs in the model grasses Setaria viridis (SvBAHD01) and Brachypodium distachyon (BdBAHD01) and determined effects on AX feruloylation. Silencing of SvBAHD01 in Setaria resulted in a c. 60% decrease in AX feruloylation in stems consistently across four generations. Silencing of BdBAHD01 in Brachypodium stems decreased feruloylation much less, possibly due to higher expression of functionally redundant genes. Setaria SvBAHD01RNAi plants showed: no decrease in total lignin, approximately doubled arabinose acylated by p-coumarate, changes in two-dimensional NMR spectra of unfractionated cell walls consistent with biochemical estimates, no effect on total biomass production and an increase in biomass saccharification efficiency of 40-60%. We provide the first strong evidence for a key role of the BAHD01 gene in AX feruloylation and demonstrate that it is a promising target for improvement of grass crops for biofuel, biorefining and animal nutrition applications. (AU) | |
| Processo FAPESP: | 16/07926-4 - Novas estratégias integrativas através de uma aldoketo redutase do cupim Coptotermes gestroi para sacarificação, detoxificação e fermentação de lignocelulose visando a produção de bioetanol |
| Beneficiário: | Robson Tramontina |
| Linha de fomento: | Bolsas no Brasil - Doutorado |