Triploid or hybrid tetra: Which is the ideal sterile host for surrogate technology?

Autor(es)

Piva, Lucas Henrique ^{[1, 2]} ; de Siqueira-Silva, Diogenes Henrique ^{[3, 2, 4]} ; Gomes Goes, Caio Augusto ^[5] ; Fujimoto, Takafumi ^[6] ; Saito, Taiju ^[7] ; Dragone, Leticia Veroni ^[2] ; Senhorini, Jose Augusto ^[2] ; Porto-Foresti, Fabio ^[5] ; Sterman Ferraz, Jose Bento ^[3] ; Yasui, George Shigueki ^{[3, 2]}

Afiliação do(s) autor(es)

^[1] UNESP Univ Estadual Paulista, Programa Posgrad Ciencias Biol Zool, Campus Botucatu, Botucatu, SP - Brazil

^[2] Ctr Nacl Pesquisa & Conservacao Biota Aquat Conti, Pirassununga, SP - Brazil

^[3] Univ Sao Paulo, Dept Med Vet, Fac Zootecnia & Engn Alimentos, Pirassununga, SP - Brazil

^[4] UNIFESSPA Univ Fed Sul & Sudeste Para, IESB, Folha 31, Quadra 07, Lote Especial S-N Nova Maraba, BR-68507590 Maraba, Para - Brazil

^[5] UNESP Univ Estadual Paulista, Fac Ciencias, Campus Bauru, Bauru, SP - Brazil

^[6] Hokkaido Univ, Fac Fisheries Sci, 3-1-1 Minato Cho, Hakodate, Hokkaido 0418611 - Japan

^[7] Ehime Univ, South Ehime Fisheries Res Ctr, Nishiura Stn, Uchidomari, Ainan - Japan

• Tipo de documento: Artigo Científico
• Fonte: Theriogenology; v. 108, p. 239-244, MAR 1 2018.
• Citações Web of Science: 2

Resumo

This work was aimed at developing an effective procedure to obtain sterile ideal host fish in mass scale with no endogenous germ cells in the germinal epithelium, owning permanent stem-cell niches able to be colonized by transplanted germ cells in surrogate technology experiments. Thus, triploids, diploid hybrids, and triploid hybrids were produced. To obtain hybrid offspring, oocytes from a single Astyanax altiparanae female were inseminated by sperm from five males (A. altiparanae, A. fasciatus, A. schubarti, Hyphessobrycon anisitsi, and Oligosarcus pintoi). Triploidization was conducted by inhibition of the second polar body release using heat shock treatment at 40 degrees C for 2 min. At 9-months of age, the offspring from each crossing was histologically evaluated to access the gonadal status of the fish. Variable morphological characteristics of the gonads were found in the different hybrids offspring: normal gametogenesis, gametogenesis without production of gametes, sterile specimens holding germ cells, and sterile specimens without germ cells, which were considered ``ideal hosts{''}. However, only in the hybrid derived from crossing between A. altiparanae and A. fasciatus, 100% of the individuals were completely sterile. Among them 833% of the male did not present germ cells inside germinal epithelium, having only somatic cells in the gonad. The other 16.7% also presented spermatogonia inside the niches. Such a methodology allows the production of sterile host in mass scale, opening new insights for application of surrogate technologies. (C) 2017 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 10/17429-1 - Quimerismo em espécies nativas de peixes como ferramenta para a propagação mediada na aquicultura
• Beneficiário: George Shigueki Yasui
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 11/11664-1 - Quimerismo em espécies nativas de peixes como ferramenta para a propagação mediada na aquicultura
• Beneficiário: George Shigueki Yasui
• Modalidade de apoio: Bolsas no Brasil - Jovens Pesquisadores