Influence of obesity on experimental periodontitis in rats: histopathological, histometric and immunohistochemical study

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Zuza, Elizangela Partata ^{[1, 2]} ; Garcia, Valdir Gouveia ^{[3, 4]} ; Theodoro, Leticia Helena ^{[3, 4]} ; Ervolino, Edilson ^[5] ; Veloso Favero, Luiz Fernando ^[6] ; Longo, Mariellen ^{[3, 4]} ; Ribeiro, Fernando Salimon ^[6] ; Martins, Alex Tadeu ^[6] ; Spolidorio, Luis Carlos ^[7] ; Sampaio Zuanon, Jose Antonio ^[7] ; Correa de Toledo, Benedicto Egbert ^[8] ; Pires, Juliana Rico ^[6]

Afiliação do(s) autor(es)

^[1] Fluminense Fed Univ UFF, Sch Dent, Dept Periodontol, Rio De Janeiro - Brazil

^[2] Inst Saude Nova Friburgo ISNF UFF, Rua Dr Silvio Henrique Braune 22, BR-28625650 Rio De Janeiro - Brazil

^[3] Sao Paulo State Univ Unesp, Sch Dent, Dept Surg, Sao Paulo - Brazil

^[4] Sao Paulo State Univ Unesp, Sch Dent, Integrated Clin, Sao Paulo - Brazil

^[5] Sao Paulo State Univ Unesp, Sch Dent, Dept Basic Sci, Sao Paulo - Brazil

^[6] Educ Fdn Barretos Unifeb, Dept Dent, Sch Dent, Sao Paulo - Brazil

^[7] Sao Paulo State Univ UNESP, Sch Dent, Dept Physiol & Pathol, Sao Paulo - Brazil

^[8] Sao Paulo State Univ Unesp, Sch Dent, Dept Diag & Surg, Araraquara - Brazil

• Tipo de documento: Artigo Científico
• Fonte: CLINICAL ORAL INVESTIGATIONS; v. 22, n. 3, p. 1197-1208, APR 2018.
• Citações Web of Science: 6

Resumo

This study assessed the influence of obesity on the progression of ligature-induced periodontitis in rats. Forty-eight adult Wistar rats were randomly divided into two groups: the HL group (n = 24) was fed high-fat animal food to induce obesity, and the NL group (n = 24) was fed normolipidic animal food. Obesity was induced within a period of 120 days, and the induction of experimental periodontitis (EP) was subsequently performed for 30 days. The animals were euthanized after 7, 15, and 30 days, and the jaws were removed for histopathological, histometric, and immunohistochemical analyses. Tartrate-resistant acid phosphatase (TRAP), receptor activator of nuclear factor kappa beta ligand (RANKL), and osteoprotegerin (OPG) were analyzed via immunolabeling. Histological findings indicated that the inflammation was more extensive and lasted longer in the HLa{''}EP; however, advanced destruction also occurred in the NL/EP. Greater bone loss was verified in the HL/EP group (2.28 +/- 0.35) in the period of 7 days than in the NL/EP group (1.2 +/- 0.29). High immunolabeling was identified in the HL/EP group in the initial periods for RANKL and TRAP, whereas the NLa{''}EP group presented with moderate immunolabeling for both factors. The HL/EP and NL/EP groups showed low immunolabeling for OPG. Obesity induced by a high-fat diet influenced alveolar bone metabolism when associated with experimental periodontitis and caused a more severe local inflammatory response and alveolar bone loss. Obesity is related to greater alveolar bone loss and an accentuated local inflammatory response, which may be reflected in the clinical severity of periodontitis and dental loss. (AU)

• Processo FAPESP: 11/08053-0 - Influência da obesidade na progressão e severidade da doença periodontal experimentalmente induzida em ratas: avaliação histomorfométrica e imunohistoquímica
• Beneficiário: Elizangela Partata Zuza
• Modalidade de apoio: Auxílio à Pesquisa - Regular