An insight into molecular motions and phase composition of gliadin/glutenin glycerol blends studied by C-13 solid-state and H-1 time-domain NMR

Autor(es)

Andrade, Fabiana Diuk ^[1] ; Newson, William R. ^[2] ; Bernardinelli, Oigres Daniel ^[1] ; Rasheed, Faiza ^[2] ; Cobo, Marcio Fernando ^[1] ; Plivelic, Tomas S. ^[3] ; deAzevedo, Eduardo Ribeiro ^[1] ; Kuktaite, Ramune ^[2]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Fis Sao Carlos, CP 369, BR-13660970 Sao Carlos, SP - Brazil

^[2] Swedish Univ Agr Sci, Dept Plant Breeding, POB 101, SE-23053 Alnarp - Sweden

^[3] Lund Univ, MAX Lab 4, Fotongatan 2, SE-22592 Lund - Sweden

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF POLYMER SCIENCE PART B-POLYMER PHYSICS; v. 56, n. 9, p. 739-750, MAY 1 2018.
• Citações Web of Science: 2

Resumo

Monitoring of the molecular motions and secondary structures of gliadin (Gli) and glutenin (Glu) in blends with 10, 20, 30, and 40% glycerol was performed by solid-state (SS) and time domain (TD) NMR spectroscopy. Increasing the glycerol content increased the relative amount of -sheets and disordered structures, while decreasing -helices in Gli/Glu-glycerol blends studied by C-13 CPMAS NMR. For 20% glycerol samples, the protein side-chain mobility increased similarly for Gli and Glu. A higher proportion of -helices versus -sheets was found in Gli-glycerol blends compared with Glu-glycerol blends. Glycerol acted as immobilized in 10-20% glycerol Gli samples and was found mainly free in 30 and 40% glycerol Gli/Glu samples. During temperature experiments, 30 and 40% glycerol amounts impacted the dynamic molecular behavior of the Gli and Glu proteins differently than lipids, as observed by TD-NMR. The combination of TD-NMR together with SS-NMR showed details of the dynamic molecular variations in Gli/Glu protein structure and are promising techniques to monitor the molecular dynamics of plasticized proteins. (c) 2018 Wiley Periodicals, Inc. J. Polym. Sci., Part B: Polym. Phys. 2018, 56, 739-750 (AU)

• Processo FAPESP: 11/14099-3 - Uso de RMN no domínio do tempo para caracterização de sistemas orgânicos de alta viscosidade
• Beneficiário: Fabiana Diuk de Andrade
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado