Engineering xylose metabolism for production of polyhydroxybutyrate in the non-model bacterium Burkholderia sacchari

Autor(es)

Guaman, Linda P. ^{[1, 2, 3]} ; Barba-Ostria, Carlos ^{[3, 4, 5]} ; Zhang, Fuzhong ^[5] ; Oliveira-Filho, Edmar R. ^[3] ; Gomez, Jose Gregbrio C. ^[3] ; Silva, Luiziana F. ^[3]

Afiliação do(s) autor(es)

^[1] Tech Univ Ambato, Fac Sci & Food Engn, Ambato - Ecuador

^[2] Univ Tecnol Equinoccial, Fac Ciencias Salud Eugenio Espejo, Ctr Invest Biomed, Quito - Ecuador

^[3] Univ Sao Paulo, Inst Biomed Sci, Dept Microbiol, Av Prof Lineu Prestes 1374 Lab 148, Cidade Univ, BR-05508888 Sao Paulo, SP - Brazil

^[4] Tech Univ Ambato, Fac Hlth Sci, Ambato - Ecuador

^[5] Washington Univ, Dept Energy Environm & Chem Engn, St Louis, MO 63130 - USA

• Tipo de documento: Artigo Científico
• Fonte: Microbial Cell Factories; v. 17, MAY 15 2018.
• Citações Web of Science: 3

Resumo

Background: Despite its ability to grow and produce high-value molecules using renewable carbon sources, two main factors must be improved to use Burkholderia sacchari as a chassis for bioproduction at an industrial scale first, the lack of molecular tools to engineer this organism and second, the inherently slow growth rate and poly-3-hydroxybutyrate {[}P(3HB)] production using xylose. In this work, we have addressed both factors. Results: First, we adapted a set of BglBrick plasmids and showed tunable expression in B. sacchari. Finally, we assessed growth rate and P(3HB) production through overexpression of xylose transporters, catabolic or regulatory genes. Overexpression of xylR significantly improved growth rate (55.5% improvement), polymer yield (77.27% improvement), and resulted in 71% of cell dry weight as P(3HB). Conclusions: These values are unprecedented for P(3HB) accumulation using xylose as a sole carbon source and highlight the importance of precise expression control for improving utilization of hemicellulosic sugars in B. sacchari. (AU)

• Processo FAPESP: 10/51989-4 - Análise sistêmica, engenharia metabólica e avaliação econômica da utilização de hidrolisados hemicelulósicos em bioprocessos
• Beneficiário: Luiziana Ferreira da Silva
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Regular
• Processo FAPESP: 16/00842-0 - Melhoramento da eficiência bacteriana no uso de xilose e co-substratos para produzir biopolímeros a partir de biomassa: engenharia da linhagem, otimização de processo e testes de aplicações
• Beneficiário: Luiziana Ferreira da Silva
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Regular