Surubim-do-Paraiba oocytes viability after being exposed to different cryoprotectants

Autor(es)

Lopes, Tais da Silva ^[1] ; Sanches, Eduardo Antonio ^[2] ; Caneppele, Danilo ^[3] ; Romagosa, Elizabeth ^[1]

Afiliação do(s) autor(es)

^[1] SAA, Inst Pesca, APTA, Av Francisco Matarazzo 455, BR-05001970 Sao Paulo, SP - Brazil

^[2] Univ Estadual Paulista Julio Mesquita Filho UNESP, Ctr Aquicultura CAUNESP, Curso Engn Pesca, Campus Expt Registro CERe, Registro, SP - Brazil

^[3] Estacao Hidrobiol & Aquicultura CESP, Paraibuna, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Ciência Rural; v. 48, n. 6 2018.
• Citações Web of Science: 0

Resumo

To know the non-toxic cryoprotectants to fish oocytes is of extreme importance for tests that aim to increase oocyte resistance to cold, thus allowing more advanced studies in cryopreservation. Therefore, commonly used cryoprotectants such as methanol, dimethyl sulfoxide, ethylene glycol, propylene glycol, sucrose and fructose were studied. Immature oocytes from the initial to vitelogenic (diameter <1.7 mm) and mature (diameter >1.8 mm) stages of Steindachneridion parahybae were evaluated. Four distinct experiments were performed, three using immature oocytes and one using oocytes at the mature stage. For each oocyte stage, the best maintenance solution to be used: Hank or 50% L15 and; viability after baths for 30min (room temperature) at cryoprotectant concentrations ranging from 0.25 to 4M were evaluated. Different tests were used to evaluate oocyte viability: in vitro maturation followed by observation of germinal vesicle breakdown (only for immature oocytes), Trypan Blue staining (all stages) and fertilization and hatching rates (mature stage only). Results showed that the toxic effect of cryoprotectants on oocytes generally increases with increasing concentrations. Sensitivity of oocytes to cryoprotectants increases according to the stage of development, with mature oocytes being more sensitive. Sucrose, fructose, methanol, propylene glycol and dimethyl sulfoxide can be used as cryoprotectants for S. parahybae oocytes. (AU)

• Processo FAPESP: 14/21215-8 - Criopreservação de ovócitos de surubim-do-Paraíba (Steindachneridion parahybae)
• Beneficiário: Taís da Silva Lopes
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado