LLLT Effects on Oral Keratinocytes in an Organotypic 3D Model

Autor(es)

Basso, Fernanda G. ^[1] ; Pansani, Taisa N. ^[1] ; Soares, Diana G. ^[1] ; Hebling, Josimeri ^[1] ; de Souza Costa, Carlos Alberto ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Araraquara Sch Dent, Araraquara - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Photochemistry and Photobiology; v. 94, n. 1, p. 190-194, JAN-FEB 2018.
• Citações Web of Science: 2

Resumo

Several invitro studies evaluated the cellular and molecular events related to interactions between phototherapy and target tissues, including oral keratinocytes and fibroblasts, providing elucidative data about phototherapy-induced healing. However, these interactions were limited to the application of a bidimensional cell culture model of oral mucosal cells. Thus, thisstudy evaluated the use of an organotypic oral epithelium model to elucidate the morphological and phenotypic responses of cells subjected to low-level laser therapy (LLLT). Oral keratinocytes were seeded in the exvivo-produced oral mucosal equivalent (EVPOME) model, with a porcine acellular dermal matrix. LLLT was applied by means of the LaserTABLE device (780 nm, 25 mW) at 0.5, 1.5 and 3 J cm(-2). After three irradiations, morphology, proliferation and gene expression of growth factors were assessed. LLLT and control groups presented similar morphological features, characterized by the formation of a stratified, differentiated and keratinized epithelium. LLLT enhanced the cell proliferation and gene expression of keratinocytes (hKGF) as well as epidermal (hEGF) growth factors. In general, analysis of these data shows that the three-dimensional cell culture model can be applied for phototherapy studies and that the positive effects of LLLT were confirmed by the use of an organotypic model. (AU)

• Processo FAPESP: 14/06057-7 - Desenvolvimento de modelo de cultura de células 3D de mucosa oral para estudos in vitro
• Beneficiário: Fernanda Gonçalves Basso
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado
• Processo FAPESP: 13/05879-0 - Efeito da laserterapia de baixa potência e fatores de crescimento sobre a atividade de fibroblastos de gengiva obtidos de pacientes jovens e idosos
• Beneficiário: Carlos Alberto de Souza Costa
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 12/17947-8 - Efeito do laser de baixa intensidade sobre a expressão de citocinas inflamatórias na mucosite oral
• Beneficiário: Fernanda Gonçalves Basso
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado