How the interaction of PVP-stabilized Ag nanoparticles with models of cellular membranes at the air-water interface is modulated by the monolayer composition

Autor(es)

Cruz Gomes da Silva, Rafael Leonardo ^[1] ; Oliveira da Silva, Heloiza Fernanda ^[2] ; da Silva Gasparotto, Luiz Henrique ^[2] ; Caseli, Luciano ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Dept Chem, Diadema, SP - Brazil

^[2] Univ Fed Rio Grande do Norte, Inst Chem, Grp Biol Chem & Chemometr, BR-59072970 Natal, RN - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Colloid and Interface Science; v. 512, p. 792-800, FEB 15 2018.
• Citações Web of Science: 7

Resumo

The antimicrobial property of silver nanoparticles (AgNPs) is believed to be associated to their interaction with biointerfaces such as microbial cell membranes, encouraging research on the identification of membrane sites capable of AgNPs binding. Although significant progress has been made in that regard, the exact molecular mechanism of action is yet to be fully understood. In this study, AgNPs dispersed in aqueous media and stabilized with polyvinylpyrrolidone were incorporated in Langmuir monolayers of selected lipids that served as cell membrane models. Results from pressure-area isotherms, vibrational spectroscopy and Brewster angle microscopy revealed condensation of glycoside-free lipid monolayers, evidencing that the AgNPs interact mostly with the lipid hydrophilic head groups. In contrast, the monolayers of systems containing glycoside derivatives were found to expand upon AgNPs incorporation, indicating that the glycosidic compounds might facilitate the incorporation of these nanoparticles in cellular membranes. These data can be therefore correlated with the possible toxicity and microbicide effect of AgNPs in lipidic surfaces of mammalian and microbial membranes. (C) 2017 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 15/09586-3 - Interação de nanopartículas de prata e ouro em filmes de Langmuir como modelos de membrana
• Beneficiário: Rafael Leonardo Cruz Gomes da Silva
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica