Sperm traits on in vitro production (IVP) of bovine embryos: Too much of anything is good for nothing

Autor(es)

Perez Siqueira, Adriano Felipe ^[1] ; de Castro, Leticia Signori ^[1] ; de Assis, Patricia Monken ^[1] ; Bicudo, Luana de Cassia ^[1] ; Mendes, Camilla Mota ^{[1, 2]} ; Nichi, Marcillio ^[3] ; Visintin, Jose Antonio ^[2] ; Ortiz D'Avila Assumpcao, Mayra Elena ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Vet Med & Anim Sci, Dept Anim Reprod, Lab Spermatozoa Biol, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Sch Vet Med & Anim Sci, Dept Anim Reprod, Lab Vitro Fertilizat Cloning & Anim Transgenesis, Sao Paulo - Brazil

^[3] Univ Sao Paulo, Sch Vet Med & Anim Sci, Dept Anim Reprod, Lab Androl, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PLoS One; v. 13, n. 7 JUL 10 2018.
• Citações Web of Science: 1

Resumo

Sperm samples used on fertilization strongly influence the in vitro production (IVP) rates. However, sperm traits behind this effect are not stated consistently until now. This study aimed to evaluate the isolated and combined effect of some sperm traits (MB: total motility before Percoll (R) gradient, MA: total motility after Percoll (R) gradient, Al: acrosome integrity, MI: membrane integrity, MP: mitochondrial membrane potential, and CR: chromatin resistance) on IVP rates. This is the first study focusing on the isolated effect of distinct traits. For this purpose, the experiment was divided in three steps. In first step, to study behavior of traits sperm samples (n = 63 batches) were analyzed and ranked based on each trait. In second step, samples ranked were selected from target ranks regions and allocated in groups of four to five batches, creating Higher and Lower groups, according to two different approaches. One aimed to form groups that differed to all sperm traits simultaneously (effect of combined traits). The other aimed to form groups that differed only to a single sperm trait while no differences were observed for the remaining traits (effect of each isolated trait). In third step, for each group successfully formed in step 2, sperm samples were individually and prospectively used for IVP. Cleavage, embryo development and blastocyst rates were recorded and compared between Higher and Lower of respective trait groups. Surprisingly, evaluation of isolated effects revealed that lower levels of MB, Al and MP resulted in higher embryo development and blastocyst rates (p<0.05), which was not observed on cleavage rate. We conclude that sperm traits strongly influence embryo development after in vitro fertilization (IVF), affecting the zygote competence to achieve blastocyst stage. Individually, levels of MB, Al or MP could be some of the key traits that may define IVP efficiency on current systems of embryo production. (AU)

• Processo FAPESP: 13/07940-9 - Efeito de atributos espermáticos na produção in vitro de embriões e na qualidade embrionária em bovinos.
• Beneficiário: Adriano Felipe Perez Siqueira
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 16/15147-5 - Uso dos atributos espermáticos para criação de um modelo preditivo para produção in vitro de embriões bovinos
• Beneficiário: Mayra Elena Ortiz D' Avila Assumpcao
• Modalidade de apoio: Auxílio à Pesquisa - Regular