Circadian Entrainment in Arabidopsis by the Sugar-Responsive Transcription Factor bZIP63

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Frank, Alexander ^[1] ; Matiolli, Cleverson C. ^[2] ; Viana, Americo J. C. ^[2] ; Hearn, Timothy J. ^[1] ; Kusakina, Jelena ^{[3, 4]} ; Belbin, Fiona E. ^[3] ; Newman, David Wells ^[2] ; Yochikawa, Aline ^{[5, 3]} ; Cano-Ramirez, Dora L. ^[3] ; Chembath, Anupama ^{[3, 6]} ; Cragg-Barber, Kester ^{[3, 1]} ; Haydon, Michael J. ^{[1, 7]} ; Hotta, Carlos T. ^[8] ; Vincentz, Michel ^[2] ; Webb, Alex A. R. ^[1] ; Dodd, Antony N. ^[3]

Afiliação do(s) autor(es)

^[1] Univ Cambridge, Dept Plant Sci, Cambridge CB2 3EA - England

^[2] Univ Estadual Campinas, Inst Biol, Dept Biol Vegetal, Ctr Biol Mol & Engn Genet, CP 6010, BR-13083875 Campinas, SP - Brazil

^[3] Univ Bristol, Sch Biol Sci, Bristol BS8 1TQ, Avon - England

^[4] Univ Leeds, Fac Biol Sci, Leeds LS2 9JT, W Yorkshire - England

^[5] Univ Estadual Campinas, Campinas, SP - Brazil

^[6] Aston Univ, Sch Life & Hlth Sci, Birmingham B4 7ET, W Midlands - England

^[7] Univ Melbourne, Sch BioSci, Parkville, Vic 3010 - Australia

^[8] Univ Sao Paulo, Inst Quim, Dept Bioquim, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Current Biology; v. 28, n. 16, p. 2597+, AUG 20 2018.
• Citações Web of Science: 7

Resumo

Synchronization of circadian clocks to the day-night cycle ensures the correct timing of biological events. This entrainment process is essential to ensure that the phase of the circadian oscillator is synchronized with daily events within the environment {[}1], to permit accurate anticipation of environmental changes {[}2, 3]. Entrainment in plants requires phase changes in the circadian oscillator, through unidentified pathways, which alter circadian oscillator gene expression in response to light, temperature, and sugars {[}4-6]. To determine how circadian clocks respond to metabolic rhythms, we investigated the mechanisms by which sugars adjust the circadian phase in Arabidopsis {[}5]. We focused upon metabolic regulation because interactions occur between circadian oscillators and metabolism in several experimental systems {[}5, 7-9], but the molecular mechanisms are unidentified. Here, we demonstrate that the transcription factor BASIC LEUCINE ZIPPER63 (bZIP63) regulates the circadian oscillator gene PSEUDO RESPONSE REGULATOR7 (PRR7) to change the circadian phase in response to sugars. We find that SnRK1, a sugar-sensing kinase that regulates bZIP63 activity and circadian period {[}10-14] is required for sucrose-induced changes in circadian phase. Furthermore, TREHALOSE-6-PHOSPHATE SYNTHASE1 (TPS1), which synthesizes the signaling sugar trehalose-6-phosphate, is required for circadian phase adjustment in response to sucrose. We demonstrate that daily rhythms of energy availability can entrain the circadian oscillator through the function of bZIP63, TPS1, and the KIN10 subunit of the SnRK1 energy sensor. This identifies a molecular mechanism that adjusts the circadian phase in response to sugars. (AU)

• Processo FAPESP: 15/06260-0 - Relógios biológicos específicos em órgãos e tecidos de gramíneas C4
• Beneficiário: Carlos Takeshi Hotta
• Linha de fomento: Auxílio à Pesquisa - Programa BIOEN - Regular
• Processo FAPESP: 08/52071-0 - Homeostase energética e sinalização por açúcar: diversificação dos mecanismos moleculares envolvidos no controle do balanço energético em angioespermas
• Beneficiário: Michel Georges Albert Vincentz
• Linha de fomento: Auxílio à Pesquisa - Programa BIOEN - Temático