Short- and long-term salinity challenge, osmoregulatory ability, and (Na+, K+)-ATPase kinetics and alpha-subunit mRNA expression in the gills of the thinstripe hermit crab Clibanarius symmetricus (Anomura, Diogenidae)

Autor(es)

Faleiros, Rogerio O. ^{[1, 2]} ; Garcon, Daniela P. ^{[1, 3]} ; Lucena, Malson N. ^{[1, 4]} ; McNamara, John C. ^{[5, 6]} ; Leone, Francisco A. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Quim, Ave Bandeirantes 3900, BR-14040901 Ribeirao Preto, SP - Brazil

^[2] Univ Fed Jatai, Unidade Acad Especial Ciencias Biol, BR-75801615 Jatai, Go - Brazil

^[3] Univ Fed Triangulo Mineiro, BR-38280000 Iturama, MG - Brazil

^[4] Univ Fed Mato Grosso do Sul, Inst Biociencias, BR-79070900 Campo Grande, MS - Brazil

^[5] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Biol, BR-14040901 Ribeirao Preto, SP - Brazil

^[6] Univ Sao Paulo, Ctr Biol Marinha, BR-11000600 Sao Sebastiao, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY; v. 225, p. 16-25, NOV 2018.
• Citações Web of Science: 4

Resumo

The evolutionary history of the Crustacea reveals ample adaptive radiation and the subsequent occupation of many osmotic niches resulting from physiological plasticity in their osmoregulatory mechanisms. We evaluate osmoregulatory ability in the intertidal, thinstripe hermit crab Clibanarius symmetricus after short-term exposure (6 h) or long-term acclimation (10 days) to a wide salinity range, also analyzing kinetic behavior and alpha-subunit mRNA expression of the gill (Na+, K+)-ATPase. The crab strongly hyper-regulates its hemolymph at 5 and 15 parts per thousand S (Salinity, g L-1) but weakly hyper-regulates up to 27 parts per thousand S. After 6h exposure to 359 , 60S and 45 parts per thousand S, C. symmetricus slightly hypo-regulates its hemolymph, becoming isosmotic after 10 days acclimation to these salinities. (Na+, K+)-ATPase specific activity decreases with increasing salinity for both exposure periods, reflecting physiological adjustment to isosmoticity. At low salinities, the gill enzyme exhibits a single, low affinity ATP binding site. However, at elevated salinities, a second, high affinity, ATP binding site appears, independently of exposure time. (Na+, K+)-ATPase alpha-subunit mRNA expression increases only after 10 days acclimation to 5 parts per thousand S. Our findings suggest that hemolymph hyper-regulation is effected by alterations in enzyme activity during shortterm exposure, but is sustained by increased mRNA expression during long-term acclimation. The decrease in gill (Na+, K+)-ATPase activity seen as a consequence of increasing salinity appears to underlie biochemical adjustments to hemolymph isosmoticity as hypo-regulatory ability diminishes. (AU)

• Processo FAPESP: 10/17534-0 - Papel da (Na,K)-ATPase na osmorregulação e excreção de amônio em crustáceos eurialinos: identificação e efeitos do FXYD2 na regulação da (Na,K)-ATPase branquial do siri Callinectes danae
• Beneficiário: Francisco de Assis Leone
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/24252-9 - O papel da (Na+,K+)-ATPase na capacidade osmorregulatória do caranguejo Ucides cordatus: uma abordagem bioquímica e molecular
• Beneficiário: Malson Neilson de Lucena
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 16/25336-0 - Estudo bioquímico comparativo da (Na+, K+)-ATPase branquial de Macrobrachium amazonicum (Heller, 1862) habitantes de regiões costeiras e continentais
• Beneficiário: Francisco de Assis Leone
• Modalidade de apoio: Auxílio à Pesquisa - Regular