MALDI-Biotyper as a tool to identify polymer producer bacteria

Autor(es)

Karolski, Bruno ^{[1, 2]} ; Cardoso, Leticia O. B. ^[2] ; Gracioso, Louise H. ^{[1, 2]} ; Nascimento, Claudio A. O. ^[1] ; Perpetuo, Elen A. ^[3]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Chem Engn Dept, POLI USP, Lineu Prestes Ave 580, Sao Paulo, SP - Brazil

^[2] Univ Sao Paulo, Environm Res & Educ Ctr, CEPEMA POLL USP, Conego Domen Rangoni Rd, 270 Km, Cubatao, SP - Brazil

^[3] Univ Fed Sao Paulo, Dept Marine Sci, Imar UNIFESP, Carvalho de Mendonca Ave 144, Santos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Microbiological Methods; v. 153, p. 127-132, OCT 2018.
• Citações Web of Science: 1

Resumo

The methylotrophs bacteria can use methane and methanol as carbon sources to produce biopolymers including the polyhydroxybutyrate (PHB) a very promised substitute for the environment contaminant oil-derived poly-propylene. This kind of bacteria can be very effective to help to decrease PHB price production and promote its use in substitution of several environment contaminant plastics. The search for methylotroph bacteria able to produce PHB is a very arduous job being necessary to grow all isolates and submit all of them to extraction processes and product characterization. Looking for time reducing and optimization of resources, we tested the Matrix Assisted Laser Desorption/lonization technique (MALDI-Biotyper) to identify polymer producer bacteria based on a single isolated colony with success. The results showed here will contribute to speed-up and increase the discoveries of new bacteria strains able to produce PHB and other biopolymers. (AU)

• Processo FAPESP: 13/50218-2 - CEPEMA - Centro Cooperativo em Engenharia Ambiental
• Beneficiário: Claudio Augusto Oller do Nascimento
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 14/50279-4 - Brasil Research Centre for Gas Innovation
• Beneficiário: Julio Romano Meneghini
• Modalidade de apoio: Auxílio à Pesquisa - Programa Centros de Pesquisa em Engenharia