Gold-based nanospheres and nanorods particles used as theranostic agents: An in vitro and in vivo toxicology studies

Autor(es)

Cancino-Bernardi, J. ^[1] ; Marangoni, V. S. ^{[1, 2]} ; Besson, J. C. F. ^[3] ; Cancino, M. E. C. ^[3] ; Natali, M. R. M. ^[3] ; Zucolotto, V ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Phys Inst Sao Carlos, Nanomed & Nanotoxicol Grp, Ave Saocarlense 400, CP 369, BR-13560970 Sao Carlos, SP - Brazil

^[2] Univ Presbiteriana Mackenzie MackGraphe, Rua Consolacao, 930 Higienopolis, BR-01302907 Sao Paulo, SP - Brazil

^[3] Univ Estadual Maringa, Morphol Sci Dept, BR-87020900 Maringa, Parana - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Chemosphere; v. 213, p. 41-52, DEC 2018.
• Citações Web of Science: 4

Resumo

The adverse effect of gold-based nanoparticles is still an open question since it depends on several factors as shape, surface charge or route of administration. In this study, we investigated the influence of shape and human serum albumin (HSA) coating on the adverse effects of spherical (AuNP) and nanorods (AuNR) gold-based particles. F C3H (fibroblast) and HTC (hepatocellular carcinoma) cell lines both from liver were exposed to 25, 75 and 125 jig mL(-1), which correspond to 109 NP mL(-1). For in vivo studies, Wistar rats received these materials by oral administration in doses of 10 jig kg(-1) or 40 jig kg(-1). Systemic toxicity was verified after 24 h and 48 h by morphological analysis, blood parameters and myeloperoxidase enzyme activity. Our results revealed that HSA corona does not influence totally the pathway of interactions between AuNP and AuNR. In vitro results evidenced that AuNP can decrease in at least 50% viability of F C3H and cell adhesion of HTC, but corona significantly overcomes these effects. No differences between shape or corona were observed in function of cell lines. In vivo studies showed that 40 tg kg(-1) of AuNP-HSA caused an enhancement of the myeloperoxidase response indicating inflammatory processes. An increase from 40% to 80% on alkaline phosphatase levels were found for all groups. Our findings suggested that gold-based particles coated or not with HSA do not cause expressive adverse effects on in vitro or in vivo systems, and their oral administration cannot cause a systemic effect in the experimental conditions used here. (C) 2018 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 12/03570-0 - Nanomateriais aplicados em nanomedicina: desenvolvimento de modelos de membrana e estudos in vitro para avaliação da toxicidade.
• Beneficiário: Juliana Cancino Bernardi
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado